Expression Of MiR-200-3p And MiR-30a-5p In Serum Of Patients With IgA Nephropathy And Its Clinical Significance

Objective: To study the level of mi R-200-3p and miR-30a-5p in serum of patients with IgA nephropathy,and explore the relationship between the expression of miR-200-3p,miR-30a-5p in serum and the extent of IgA nephropathy.Methods:24 patients with primary IgA nephropathy diagnosed as renal biopsy from August 2016 to December 2016 in the First Affiliated Hospital of Dalian Medical University were recruited as the case group.According to Lee's pathological standard we divided them as Group A(I~II)and B(III~V).Selected 12 people as the health control(HC),who was no statistically significant differernce in gender and age.Select people's peripheral blood in the morning,Then separate them with the centrifugal machine.Total RNA was extracted from serum using Trizol solution.The concentration and purity of total RNA samples were measured by NanoDrop2000 spectrophotometer.We use real-time PCR to quantify the expression of miRNAs.RNU6 B was used as house-keeping genes to normalize the miRNA expression.The data was analyzed by 2^-??CT relative quantification method.To explore the relation between the expression miRNA in serum of patients with IgA nephropathy,and the correlation between miRNAs and clinical parameters,including serum creatinine,blood urea nitrogen,serum uric acid,serum albumin,eGFR,IgA1,24 h urinary protein.The values in this study which met the normal distribution were presented as x±s.The differences between groups were performed using t test and ANOVA.If they didn't meet the normal distributions,they were presented as median(interquartile range).And they were performed using Mann-Whitney U test.If the value didn't meet homogeneity test of variance,we performed them by using Dunnett's T3 test.The correlation was calculated using Spearman rank correlation.All statistical tests were performed using SPSS 17.0.P values less than 0.05 were considered significant.P values less than 0.01 were considered statistically significant.Results: 1.Compare the sitation of baseline in Group A,Group B and the healthy control group(HC).The ages,gender,blood glucosehad,cholesterol,triglycerides,low decsity lipoprotein,high decsity lipoprotein,not significant differences among three groups(P >0.05).The concentration of Blood urea nitrogen(BUN),Serum Uric Acid(UA)and serum creatinine(Scr)between group A and HC had not significant differences(P >0.05),and the concentration of BUN,UA,Scr in group B were higher than it in group A and group HC(P <0.05).The serum albumin and eGFR in group A and group B were lower than it in group HC(P <0.05),but there were no significant difference between group A and B(P > 0.05).The difference of IgA1 between Group A and Group B also had no significant differences(P >0.05).The 24 h urine protein quantity in group A was lower than it in group B(P <0.05).2.Compare the expression of serum miR-200-3p in Group A,Group B and Group HC.The levels of serum miR-200-3p in Group A and Group B were both lower than Group HC(P <0.05).And the level of serum miR-200-3p in Group B was significantly lower than Group A(P <0.01).3.Compare the expression of serum miR-30a-5p in Group A,Group B and Group HC.The levels of serum miR-30a-5p in Group A and Group B were both higher than Group HC(P <0.01).And the level of serum mi R-30 a in Group B was significantly higher than Group A(P <0.01).4.The relationship between the expression of miR-200-3p in serum and the Oxford classification(M/E/S/T).There were no relationship between the levels of serum miR-200-3p and M(P > 0.05).Serum expression of miR-200-3p were negatively correlated with/S/T(P <0.05),5.The relationship between the expression of miR-30a-5p in serum and the Oxford classification(M/E/S/T).Serum expression of miR-30a-5p were postively correlated withM/S/T(P <0.05),6.The relationship between the expression of miR-200-3p in serum and the clinical characters.There were no relationship between the levels of serum mi R-200-3p and the BUN(r =-0.125,P =0.547),Scr(r =-0.429,P =0.076),serum albumin(r=0.547,P =0.152),eGFR(r=0.311,P =0.180),IgA1(r=-0.375,P =0.125),24 h urine protein quantity(r=-0.318,P =0.199).Serum expression of miR-200-3p were negatively correlated with UA(r=-0.716,P =0.01),7.The relationship between the expression of miR-30a-5p in serum and the clinical characters.There were no relationship between the levels of serum miR-30a-5p and the BUN(r = 0.401,P =0.052),Scr(r = 0.382,P =0.065),serum albumin(r=-0.252,P =0.234),serum expression of miR-30a-5p were postively correlated with 24 h urine protein quantity(r=-0.545,P =0.006),UA(r=0.430,P =0.036),IgA1(r=0.492,P =0.015),and were negatively correlated with eGFR(r=-0.422,P =0.040).Conclusion: 1.The expression of serum miR-200-3p in patients with IgA nephropathy was decreased,and the pathological damage was heavier,the expression was lower.In addition,the expression of miR-200-3p was negatively correlated with glomerulosclerosis,renal interstitial fibrosis and serum uric acid,2.The expression of serum mi R-30a-5p in patients with IgA nephropathy was increased,and the pathological damage was heavier,the expression was higher.In addition,the expression of miR-30a-5p was postively correlated with glomerulosclerosis,renal interstitial fibrosis,24 h urine protein quantity,eGRF,serum uric acid.