The Mechanism Research Of Let-7a In Hep-2by Regulating HMGA2

Objective: laryngeal squamous cancer(LSC),which with high incidence,is one of the most common malignant tumors in respiratory.Therefore,it is important to study the development and progression mechanism of LC for improving curative effects of patients.Highmobility group A2(HMGA2),as a small non-histone chromosomal protein,was highly expressed in many human tumors and associated with poor prognosis,but the role of HMGA2 in lC remained unclear.The microRNAs(miRNA),is a family of mature noncoding small RNA.Let-7a,as a member of miRNA family,by binding to the 3’-untranslated regions(3’UTR)of the target mRNA,induces translational repression or mRNA degradation of many genes.However,there is no repots on the regulation of HMGA2 by let-7a in LC.In this study,basised on the previous studies,by transfected let-7a mimics into Hep-2,we also analyzed the effects of overexpressed let-7a on the expression of HMGA2 and the bioactivity of Hep-2 cell.Methods: 1.Let-7a mimics was designed and synthesized,and transfected into HepG2 cells.Then,detected the cel l transfection rate.2.After tranfected successfully,Real-time qPCR was used to detect the expression level of let-7a.3.Real-time qPCR and western blot were used to detect the effects of overexressed let-7a on HMGA2 expression.4.In vitro,cell proliferation and apoptosis were assessed by flow cytometry.Results:1.After let-7a tranfected successfully,Real-time qPCR prompted that the expression level of let-7a in the experimental group were higher than the blank group and the negative control(NC)group,The difference have statistically significant(P<0.05);The difference between the blank group and the negative control(NC)group have no statistically significant(P>0.05).2.After let-7a tranfected successfully,Real-time qPCR prompted that the expression level of HMGA2 mRNA in the experimental group were less than the blank group and the negative control(NC)group,The difference have statistically significant(P<0.05);The difference between the blank group and the negative control(NC)group have no statistically significant(P>0.05).3.After let-7a tranfected successfully,western blot prompted that the expression level of HMGA2 in the experimental group were less than the blank group and the negative control(NC)group,The difference have statistically significant(P<0.05);The difference between the blank group and the negative control(NC)group have no statistically significant(P>0.05).4.Hep-2 was followed by flow cytometry to detected the state of cell apoptosis after transfected let-7a mimics and let-7a mimics NC for 24 hours.The result prompted that the Hep-2 cells apoptosis increases after transfected let-7a mimics.The difference have statistically significant(P<0.05).Conclusions:(1)Overexpression of let-7a could inhibit expression of HMGA2 mRNA and protein in Hep-2 cells.HMGA2 maybe was one of the target gene for let-7a.(2)Overexpressed let-7g could inhibit the proliferation and migration of Hep-2 cells.