Effect Of RNA Interference Of IONS Gene Silencing On Proliferation And Apoptosis Of Human Cholangiocarcinoma Cell Line QBC939 In Vitro

Objective: This study is to design and synthesize i NOS-si RNA,transfection of human Cholangiocarcinoma cell line QBC939 in vitro,to investgate the role of i NOS on the proliferation,invasion and apoptosis in Cholangiocarcinoma cell.To Provide theoretical basis for the targeted treatment of bile duct carcinoma.Methods: QBC939 cells were conventionally cultured.This experiment was divided into 3 groups:i NOS-si RNA intervention group,control si RNA intervention group and blank control group.Immunohistochemical staining was used to detect i NOS expressed in cholangiocarcinoma cells,RT-PCR and Westrn blotting were used to detect the gene expression of i NOS,MTT assay was used to detect the cell viability,flow cytometry was used to measure the apoptosis and cell cycle of the transfected cells,transwell assay was used to determine invasion of transfected cells.Results: Human cholangiocarcinoma QBC939 cells expressed i NOS protein;RT-PCR and Westrn blotting results showed that three i NOS-si RNAs could effectively inhibit i NOSm RNA and protein expression(p<0.05),which si RNA2 silence has the most significant effect(p<0.05);MTT assay result showed the cell growth and proliferation were inhibited obviously after transfection(p<0.05);flow cytometry result displayed the transfected group can facilitate apoptpsis of QBC939 cells(p<0.05),the other groups can not(p<0.05);Transwell assay results showed that cell number of transfection i NOS-si RNA were significantly less than other groups(p<0.05)?Conclusions: RNAi can specifically inhibit i NOS expression to effectively reduce the m RNA and protein expression level of i NOS,which can inhibit the proliferation of QBC939 cells and reduced cell invasion,and promote apoptosis of QBC939 cells.