Neurons Developed In Testis For The Regeneration Of Spinal Cord Injury

Spinal cord injury(SCI)results in neural loss and consequently motor and sensory impairment below the injury.There are currently no effective therapies for the treatment of SCI in humans.Traditional surgery and drug therapy can only improve the damage,but not effectively promote the regeneration of neurons and axons.In recent years,transplantation of cells into the injured spinal cord has been shown to be an effective treatment because of their competence to differentiate into neurons and oligodendrocytes and to secrete neurotrophic factors.Researchers use cell transplants to regenerate neurons and axons for forming new neuronal circuitry to promote spinal cord regeneration and restoring spinal cord function.While,one of the most important challenges faced when trying to repair the injured spinal cord by cell transplantation is how to get the ideal source of transplanted cells for efficient and safe cell replacement.In this article,we are committed to finding an ideal cell source for a large number of spinal cord injury transplant to make cell transplantation more efficient and convenient.Recent studies have suggested that 293T cells possess the properties of neural progenitor cells,while the testis is rich in multiple nerve growth factors,and its mechanism cells have typical marker of nervous system cells,numerous studies have also shown that immune immunities in the testis make it useful as a receptor for tissue or cell transplantation.293T cells were implanted into the mouse testis and then researched cell growth and differentiation through in vivo and in vitro experimentation.at last,cultured neurons were transplanted to SCI rats for spinal cord repair.We analyzed the migration,morphology,proliferation and neuronal marker expression of these cells.293T-derived cells migrated to the whole testis on day 7,and most of the transplanted cells had obvious morphological change and differentiated into neuron-specific nuclear protein-positive(NeuN-positive)and beta ? tubulin-positive(?-?tubulin-positive)neurons within 1 week after transplantation.The experiment found that cultured in vitro,transplanted cells maintain proliferation on day 2 and day 7,but cells at 7th day no longer multiply and observered expression of NeuN and ?-? tubulin,the results proved that 293T cells implanted into the testis differentiation into neurons,and can cultured in vitro.We found that 293T cells were not able to differentiate into neurons by testis homogenate in vitro,which indicated that the differentiation of 293T cells was required in the living testis tissue,and testicular tissue microenvironment is required.We revealed that transplanted 293T cell-derived neurons contributed to regenerating new neurons,Inhibition of astrocyte proliferation and expression of glial fibrillary acidic protein(GFAP),reducing glial scar formation and restoring motor function.In summary,we have obtained a large number of neurons derived from 293T cells in testis,and achieved good results in the treatment of SCI rats.This approach can be efficient,convenient and safe to access a large number of neurons,which guarantees the cell source or cell number of transplantation and provides a new possibility for the treatment of spinal cord and other nerve injuries.