The Study Of Anti-tumor Activity Of Conyza Blinii Saponin

Natural anti-cancer drugs gradually become the focus of research,because of their low side effects,ideal biological activity and less resistency.Conyza blinii H.Lev.,commonly called Jin Long Dan Cao,is the dried aerial parts of Compositae and distributed in Yunnan,Sichuan and other provinces.It is used for the treatment of gastroenteritis,chronic bronchitis,and other inflammatory diseases.Conyza blinii saponin(CBS)is composed mainly of triterpenoidal saponins,biological saponins belong to secondary metabolites.A number of epidemiological investigations,animal experiments and in vitro experimental carried out the antitumor effect of multi-components,multi-pathway,multi-mechanism of saponins.In this experiment,the MTT assay was conducted to detect the effects of anti-tumor activities.The results suggested that CBS can inhibit the proliferation of a few tumor cell lines,especially HeLa cells.The activities of CBS against HeLa cells including apoptosis induction,cell proliferation suppression and migration inhibition were examined by DAPI staining,DNA ladder assay,flow cytometry,transwell assay and western blotting respectively.The results showed that CBS was able to block the cells in S phase and induce the apoptosis of HeLa cells,inhibit their migration and invasion.Further studies showed that CBS could promote the mRNA expression level of p53 gene,increase the mRNA expression of CDK inhibitor p21,p27 and p57,and induce tumor cell apoptosis by mitochondrial pathway through caspase-9 and caspase-3 activation.In conclusions,our studies have proved that CBS has a strong inhibitory activities against cancer cells via different mechanisms.Additionally,CBS has also been proved to have low toxicity on normal cells.Therefore,our experiments have provided both theoretical and materisl basis for developing anti-tumor agent.