Research Of ?-catenin Protein Expression In Gastric Mucosas And Analyses Of Different Chinese Medical Syndromes Of Hp Related Gastropathy

Objective:To analyze the link between different pathology of gastric mucosa and Chinese medical syndrome,discuss the express of ?-catenin protein in Hp related gastropathy in the process of canceration by test pathology of gastric mucosa and the expression of ?-catenin protein of Hp related gastropathy within different syndrome.Aim to discover the essence of Chinese medical syndrome in Hp related gastropathy,which would do good to clinical doctors.Method:1.All the specimen were patients from endoscope center and gastrointestinal surgery of the first affiliated hospital of Guangzhou university of TCM,There were 174 gastric mucosas in total,grouped to chronic gastritis(CG)group(119),peptic ulcer(PU)group(32),gastric carcinoma(GC)group(23).In terms of Chinese medical syndromes,all the subjects grouped to splenogastric hygropyrexia syndrome group(45),spleen-deficiency syndrome group(54),liver-stomach disharmony syndrome group(44),stagnant blood block syndrome group(22),The normal group(9)were volunteers.All the subjects were well informed and asked about medical history respectively by experienced doctors,then filled forms and recorded scores.2.All the gastric mucosas were under pathomorphological examination,observed inflammation indexes;Hp was detected by methylene blue staining and rapid urease test(RUT).32 Analyzed clinical data combine pathological result.4.The protein expression of P-catenin was detected by immunohistochemistry,then compared the expression of different diseases and syndromes.Results:1.General situation:There were no significant differences between groups in terms of age,course of disease,gender distribution.2.The distribution of syndromes:All the syndromes of each disease were had no significant difference.Spleen-deficiency syndrome accounted the most of CG.Splenogastric hygropyrexia syndrome accounted the most of GC and PU.3.Compared Hp infection between each disease and syndrome.Liver-stomach disharmony syndrome group had the trade higher than the other two groups,in addition,the severe infection ratio of splenogastric hygropyrexia syndrome group was higher than the other 4 groups.In terms of different diseases,Hp infection ratio in GC and PU were apparently higher than CG(P<0.05).Infection degree of GC was significant serious than CG(P<0.05).4.The relationship between gastric mucosas pathology and each disease,syndromeThe inflammation degree,inflammation activity,atrophy,intestinal metaplasia,atypical hyperplasia all had significant positively related to Hp infection degree(P<0.05).In category of splenogastric hygropyrexia syndrome group,liver-stomach disharmony syndrome group,stagnant blood block syndrome group,spleen-deficiency syndrome group,the inflammation degree decline in turn.The inflammation in GC and PU were serious apparently(P<0.05).Inflammation activity,atrophy,intestinal metaplasia and atypical hyperplasia had no significant difference between each syndrome,while the 4 indexes of GC were serious than PU and CG apparently(P<0.05).5.Comparison of clinical symptoms in different diseasesGC patients had apparent anorexia then CG(P<0.05),while gastrectasia in GC was more apparent(P>0.05).stomachache was more severe in PU than in GC and CG(P>0.05).6.Protein expression of ?-catenin in each syndromes and diseasesThere almost no ?-catenin protein expression in gland and epithalial cells patina in the normal group,while there were protein expression in the other four groups in different dergrees,especially in the Hp infection ones.The protein expression of ?-catenin had positively related to Hp infection degree,inflammation degree,atrophy,intestinal metaplasia,atypical hyperplasia(P<0.05).In terms of syndromes,the protein expression of the 4 abnormal syndromes were significant higher than the normal group(P<0.05).In the category of splenogastric hygropyrexia syndrome group,liver-stomach disharmony syndrome group,stagnant blood block syndrome group,spleen-deficiency syndrome group,the protein expression were decline in turn.The protein expression in GC was significant higher than in PU and CG(P<0.05).Conclusion:1.There are relationship between different syndromes and gastric mucosa pathology.The HP infection ratio and inflammation degree in excess syndrome(splenogastric hygropyrexia syndrome and liver-stomach disharmony syndrome)are obvious than other syndromes.And the inflammation degree,activity,atrophy,intestinal metaplasia,atypical hyperplasia in splenogastric hygropyrexia syndrome are obvious than other syndromes.2.Among Hp related gastropathy diseases,the protein expression of ?-catenin was different in Chinese medical syndromes.The protein expression in splenogastric hygropyrexia syndrome and liver-stomach disharmony syndrome were significant higher than spleen-deficiency syndrome and stagnant blood block syndrome group within Hp positive subjects.The protein expression of ?-catenin in GC is significant higher than in PU and CG.