Effects And Possible Mechanisms Of XXM And KAE On Brain Injury With Chronic Ischemia

ObjectiveTo investigate the effects and possible mechanisms of XXM and its monomer KAE in the rats with chronic cerebral ischemia.MethodsChronic cerebral hypoperfusion model is produced by permanent occlusion of bilateral common carotid arteries(2-VO)in rats.After XXM and KAE treatment,the rats underwent Morris water maze,inclined plane and prehensile traction test.Neuronal morphology was observed using Nissl and HE staining.The activity of SOD and the content of MDA in brain tissue were determined.The content of VEGF,BDNF and NT-3 in brain tissue were determined with ELISA.The DJ-1,synaptophysin,GAP,PTP,P2X7 protein expression was assayed by Western Blotting.PC12,hypoxia cell model,induced by CoC12 were treated with XXM and KAE.The DJ-1,Keapl and Nrf2 protein expression was assayed by Western Blotting.Results1.XXM and its monomer KAE could significantly increase the behavioral function.2.XXM and its monomer KAE could significantly improve the injury of chronic cerebral hypoperfusion model.ConclusionIn summary,XXM and its monomer KAE are a neuroprotective agent against the damage by chronic cerebral ischemia,making it a potential therapeutic agent for stroke treatment.