The Effects Of Buzhong Yiqi Decoction Containing Radix Astragali Or Radix Hedyseri On The Immune Function Of Spleen Lymphocytes In SAMP8 Mice

Objective: The serums were prepared after intragastric administrated mice each with Buzhong Yiqi decoction containing Radix Hedyseri or Radix Astragali and equivalent saline.Cultured spleen lymphocytes of SAMP8 mice with the serums individually.were detected to compare the anti-immunosenescence effects of the serum containing Buzhong Yiqi decoction of Radix Astragali with the serum containing Buzhong Yiqi decoction of Radix Hedyseri on splenic lymphocytes of SAMP8 mice.Methods: 90 Kunming species young mice of healthy,as much male as female,were divided into 3 groups randomly,each group 30.Both containing Radix Astragali Buzhong Yiqi decoction and containing Radix Hedyseri Buzhong Yiqi decoction were administrated by gastric perfusion of crude drugs,once a day at 4g/kg.and blank group were administrated by gastric perfusion of 0.9% saline,once a day at 0.4ml/kg,14 day.then 2 hours after the last gastric perfusion,drawing blood through ophthalmectomy,preparation of medicated serum.To determine the best drug-containing serum concentration and action time through MTT methods.Containing the Radix Astragali Buzhong Yiqi decoction group,containing the Radix Hedyseri Buzhong Yiqi decoction group,and the blank group were cultivated with SAMP8 mice spleen lymphocytes according the best drug-containing serum concentration and action time.Both the young mice group and the SAMP8 mice group using a complete culture medium containing 10% fetal bovine serum cultured respectively with spleen lymphocytes by their own kind.Testing the influence of the serums on T cell proliferation induced by ConA,Analyzing the T lymphocyte subgroup expression by flow cytometry,detecting the content of IL-2 and IFN-? in the culture supernatant with ELISA,detecting the expression of CD28 mRNA inside spleen lymphocytes with real-time fluorescence quantitative PCR,and detectting the content of CD28 protein inside spleen lymphocytes by Western-Blot.Results: MTT results showed that 40% of drug concentration with serum training 48 h stimulated proliferation best by acting on spleen lymphocyte in SAMP8 mice.Both serums improved the proliferation of T lymphocytes in SAMP8 mice;no obvious effect on differentiationof T lymphocyte subsets in SAMP8 mice,the results were not statistically significant;increased the content of IL-2 and INF-?in the supernatants of spleen lymphocytes and for the content of IL-2,the serum of Buzhong Yiqi Decoction with Radix Hedysari was better(P<0.05);improved the expression of T lymphocyte CD28 mRNA and the effect of Radix Hedysari group was better than that of Radix Astragalus group(P < 0.05);improved the expression of CD28 protein in spleen T lymphocytes of SAMP8 mice.Conclusion: the serums containing Buzhong Yiqi decoction of Radix Astragali or Radix Hedyseri improved the immune function of SAMP8 mouse spleen T lymphocytes.furthermore Radix Hedysari group was better than Radix Astragali group in increaseing T lymphocyte proliferation and promoting the secretion of IL-2 and the expression of CD28 mRNA.