"Angelica Sinensis-ligusticum Chuanxiong" Medicine Characteristics Of Metabolism In Rat Urine

During thousands of years period,Traditional Chinese medicine was prosperous for a long time in history,it has formed Chinese characteristics and self-contained Chinese medicine theory through tireless exploration of countless physicians in the long history,certain aspects of playing an irreplaceable role in Western medicine.However,our Chinese traditional medicine is facing serious challenge for the lack of canonical system of quality criterion for Chinese traditional medicine,so it has been a urgent problem to set up the quality control standards and reveal therapeutic basis of Chinese traditional medicine.In this thesis,The high performance liquid chromatography tandem mass spectrometry(HPLC-MS)based methodologies of studies on chemical substance of "Angelica sinensis-Ligusticum chuanxiong" medicine and the variation of the chemical constituents of compatibility of every medicine were constructed.We analyzed metabolites in rat urine of“Angelica sinensis-Ligusticum chuanxiong”medicine and identified compositions resouce in " Angelica sinensis-Ligusticum chuanxiong”medicine.The main contents and productions were as followed:In the first part of this thesis,we used high performance liquid chromatography tandem mass spectrometry(HPLC-MS)online,18 chromatographic peaks were marked in the liquid chromatogram of double grugs.Compared sample with standard substance solution,15 chemical constituents were identified in double grugs:?-sitosterol,Senkyunolide(H),Senkyunolide(I),Pregnenolone,Z-ligustilide,E-Butylidenephthalide,Ferulic Acid,E-ligustilide,3',6,8',3a-diligustilide,Caffeic acid,Butylphthalide,5-hydroxy-3-Aden based phthalide,Senkyunolide A,Z-Butylidenephthalide.In the second part of this thesis,we used the same methods to mark 21 chromatographic peaks in the liquid chromatogram of Double grugs.17 chemical constituents in rat urine of Double grugs were identified,containing 7 prototype constituents:?-sitosterol,M1,E-Butylidenephthalide,M2,M3,M4,Butylphthalide,M5,Senkyunolide(I/H),M6,Pregnenolone,M7,Chuanxiong triterpene,M8,3',6,8',3a-diligustilide,Z-Butylidenephthalide,M9,M10.In the third part of this thesis,The third part of the drug to the analysis of metabolic pathway in rat urine,Ligusticum chuanxiong and Angelica that same Umbelliferae,with similar chemical composition.By the HPLC-ESI/MS analysis and combined with the relevant literature,that Coffee acid and ferulic acid as main material and two shared,andmetabolites in urine werecomposedof Coffee acidandferulic acidby methylation,acetylation,reduction,hydrol ysis,glucuronidation and other chemical process.This can be speculated that drugs on the chemical components in the urine,twocontributions.All the results provide a basic data for the further studies on effective constituents and the effecting mechanism of Double grugs.