The Study Of Active Ingredient Screening Of Anemarrhena Asphodeloides On Hyperthyreosis Disease And In-vivo Metabolism

Hyperthroidism is caused by a variety of reasons,and(or)Blood circulation thyroid hormone levels increased lead to a kind of common endocrine disease.On clinical perTab.ance high metabolic syndrome group,goiter,exophthalmos,nervous and cardiovascular system dysfunctionThyroid pathology showed diffuse,nodular or mixed swelling and other symptoms.Anemarrhena from Liliaceae Anemarrhena roots,sweet,bitter,eold,lung,stomach,kidney.With heat purging fire,Health and body fluid,nourishing yin,moistening Hua Chang effect.In this paper,extraction and separation for medicinal Anemarrhena.By the method of macroporous resin and Semi-preparative liquid to preparation of chemical composition,To prepare for the pharmacological experiments.Using HPLC-QTOF-MS/MS technology research Anemarrhena water extraction metabolic objects,nineteen compounds were found.The use of Levothyroxine sodium established hyperthyroidism animal model.By animal body weight,water,food,heart weight and serological to assess the effect of sample.Thereby selecting the active ingredient.Timosaponin can simultaneously reduce weight gain,eating and drinking,and increased cardiac hypertrophy and other four indicators(P<0.05/0.01),Anemarrhena water extract can significantly reduce weight gain and cardiac hypertrophy(P<0.05),Anemarrhena xanthones can significantly reduce weight gain and cardiac hyPertrophy(P<0.05).In summary,hyperthyroidism timosaponin has the best therapeutic effect.Therefore,timosaponin After the main active ingredient in B? and B?conducted preliminary efficacy evaluation.Timosaponin B? can reduce weight gain,increased food intake and cardiac hypertrophy three indicators(P<0.05/0.01),Timosaponin B? can significantly reduce food intake and weight gain,drinking water,increased cardiac hypertrophy and serum free T4 levels on five indicators(P<0.05/0.01).The above shows timosaponin B?with hyperthyroidism treatment.Finally,mcompare the metabolic difference between the normal animals and models.Using HPLC-QTOF-MS/MS techniques,we found prototype components and 16 reactions(Dehydration,deglycosylation,hydroxylation,etc.).Animals without modeling,In plasma/ind prototype and ten metabolites.In urine,find prototype and fifteen metabolites.In Manure,find prototype and ten metabolites.In heart,liver,spleen,kidney and thyroid,find prototype and one metabolite.In lung find one metabolite.Animals with modelingjn plasma,find prototype and eight metabolites.In urine,find prototype and twelve metabolites.In Manure,find prototype and eleven metabolites.In Heart,Iiver,kidney and thyroid find prototype and one metabolite.In lung and spleen find prototype.In summary,Anemarrhena treatment hyperthyroidism is timosaponin B?.After the administration,The timosaponin B? no significant difference in timosaponin B? normal animals and animal models in vivo metabolites species,but in a slightly different tissue distribution.