Studying On Optimum Proportions Of Radix Ginseng-Radix Aconiti Lateralis Drug Pair Improves Yang-deficiency Type Of Heart Failure Rat

Objective Under the guidance of Chinese medicine theory,establishments and evaluation of yang-deficiency type of heart failure rat;Through the different proportions of Radix Ginseng-Radix Aconiti Lateralis drug pair yang-deficiency type of heart failure rat of hemodynamics and myocardial tissue pathological examination,using ELISA method to determine each rat serum T3,T4,TSH,ALD,and BNP's content,and screening of the optimal ratio of Shenfu medicine against heart failure.In order to seek the compound medicine on compatibility mechanisms of modern research methods,to elucidate the efficacy of effective components and the relationship between the therapeutic effect of Shenfu medicine compatibilityMethods1.Establishments and evaluation of yang-deficiency type of heart failure rat modelUsing the rat aortic stenosis,determined by observing the general state,weight changes,respiration,heart rate,body temperature,and the establishment of TCM syndrome diagnosis standard of yang-deficiency type of heart failure rat models,is used to evaluate whether the model isstable and reliable;2.Detection of Radix Ginseng-Radix Aconiti Lateralis drug pair extract and contentExtraction of different proportions of Shenfu medicine by water decocting method,to obtain the extract containing 1g�g-1 crude drug.Based on previous studies,using the method of HPLC was measured in the extracts from ginsenoside Rg1,Re,Rb1,mesaconitine,aconitine and hypaconitine index component content;3.Studying on optimum proportions of Radix Ginseng-Radix Aconiti Lateralis drug pair improves yang-deficiency type of heart failure rat3.1 Take the 7 day postoperative survival of yang-deficiency types of heart failure rat,according to the ratio of Shenfu medicine is divided into 1:0.1,1:0.5,1:1,1:2,1:10 five groups,set at the same time red ginseng group,aconite group,operation group and sham operation group,a total of 9 groups.According to the daily 100mg�kg-1 rats weighing intragastric administration for 42 days,operation group and sham operation group with the same volume of normal saline;3.2 Forty-third days after the administration to rats weighing,determination ofthe parameters of hemodynamics index[HR?LVSP?LVEDP?�dp/dtmax];3.3 Take heart of all rats were weighed,observed lesions,and calculation of HWI The left ventricular freed wall,fixed by 10%formaldehyde,ethanol dehydration,xylene penetration,embedded in paraffin,conventional slice(thickness 4?m),HE stained,observed under optical microscope in myocardial tissue lesions;3.4 To take all kinds of rat carotid artery blood,using ELISA method to determine the serum T1,T4,TSH,ALD,BNP levels.Results1.Operation of abdominal aorta stenosis establishde method of yang-deficiency type of heart failure rat model,the emergence of heart yang-qi deficiency symptoms and signs,the amount of activity and food intake decreased,lower the body temperature,respiratory frequency and heart rate accelerated obviously.The establishment of the TCM diagnostic criteria of yang-deficiency type of HF rat model,and successfully used for the evaluation of rat model of heart yang-deficiency;2.The different proportions of ginseng and aconite medicine using HPLC method to make the index components detection.The results show that the ginsenoside components of ginsenoside Rgi,Re,Rb1 content:?Shenfu 1:0.1 group respectively 4.89117mg�g-1?2.87235mg�g-1?5.25666mg�g-1;?Shenfu 1:0.5 group respectively 4.67170mg�g-1?2.64362mg�g-1?5.02338mg�g-1;?Shenfu 1:1 group respectively 3.78647mg�g-1?1.97884mg�g-1?4.12102mg�g-1;?Shenfu 1:2 group respectively 3.39821mg�g-1?1.83017mg�g-1?3.73337mg�g-1;?Shenfu 1:10 group respectively 1.82816mg�g-1?0.88876mg�g-1?2.15275mg�g-1;?Red ginseng group respectively 5.70939mg�g-1?3.47431mg�g-1?6.11699mg�g-1 Aconitum alkaloids components of mesaconitine,aconitine,hypaconitine content:?Using the HPLC method can not detected three Aconitum alkaloids in Shenfu 1:0.1 group;?In Shenfu 1:0.5 group,aconitine can not be detected,mesaconitine is 0.03794mg�g-1,hypaconitine is 0.04515mg�g-1;? In Shenfu 1:1 group,aconitine can not be detected,mesaconitine is 0.05137mg�g-1,hypaconitine is 0.09998mg�g-1;?Shenfu 1:2 group respectively 0.09387mg�g-1?0.04597mg�g-1?0.19844mg�g-1;?Shenfu 1:10 group respectively 0.20124mg�g-1?0.08812mg�g-1?0.50819 mg�g-1;?Aconite group respectively 0.35238mg�g-1?0.12231mg�g-1?0.78532 mg�g-1.3.Studying on optimum proportions of Radix Ginseng-Radix Aconiti Lateralis drug pair improves yang-deficiency type of heart failure rat3.1 According to TCM syndrome diagnosis standard of yang-deficiency type of HF rat model,to judge the syndrome of rats:The results show that,for the sort of the therapeutic effect of Shenfumedicine:Shenfu medicine on 1:10 group>Shenfu medicine on 1:0.5group?aconite group>Shenfu medicine on 1:1 group?Shenfu medicine on 1:2 group?red ginseng group>Shenfu medicine on 1:0.1 group;3.2 Shenfu drugs can improve blood dynamics index of yang-deficiency type of HF rats in different degree,the reduction of HR and LVEDP,increased LVSP,+dp/dt,its efficacy are ranked as follows:Shenfu medicine on 1:10 group>red ginseng group?aconite group>Shenfu medicine on 1:0.5 group>Shenfu medicine on 1:1 group?Shenfu medicine on 1:2 group>Shenfu medicine on 1:0.1 group;3.3 Effects of Shenfu medicine of heart weight indices,cardiac pathology slices can be seen,different ratio of Shenfu medicine against heart failurehas certain therape utic effect and the curative effect,the sorting is as follows:Shenfu medicine on 1:10 group>Shenfu medicine on 1:0.5 group?aconite group>Shenfu medicine on 1:1 group?Shenfu medicine on 1:2 group?red ginseng group>Shenfu medicine on 1:0.1 group;3.4 Different proportions of Shenfu medicine can effectively improve the rats serum T3,T4,TSH levels and the sorting is as follows:Shenfu medicine on 1:10 group>Shenfu medicine onl:0.5 group?aconite group?red ginseng group>Shenfu medicine on 1:1 group?Shenfu medicine on 1:2 group>Shenfu medicine on 1:0.1 group;Different proportions of Shenfu drugs could inhibit the rat serum ALD,BNP levels and the sorting is as follows:Shenfu medicine on 1:10 group?Shenfu medicine on 1:0.5 group>Shenfu medicine on 1:1 group?aconite group?red ginseng group>Shenfu medicine on 1:2 group?Shenfu medicine on 1:0.1 groupConclusion1.Successfully established the model and evaluation standard of yang-deficiency type of HF rats.The results show that,in the constriction of abdominal aorta in rats after aggravating heart failure symptoms,and embodies the "yang-deficiency" of the syndrome in TCM;The establishment of TCM syndrome diagnosis standard of yang-deficiency type of HF rat model,we set yang deficiency performance as the main symptom,and symptoms of the "heart" which reflects the organs positioning as secondary symptoms,which reflects the yang deficiency syndrome,and reflects the viscera.Rats body temperature,respiratory rate and heart rate can be quantitative index to indicate the main symptoms are more objective and scientific2.Different proportions of Shenfu drug have therapeutic effects on yang deficiency type of HF rats,among them with Shenfu medicine curative effect of 1:10group was the best.To study the mechanism of action:Shenfu medicine can improve the index of blood flow dynamics of yang deficiency type of HF rats in different degree,the reduction of HR and LVEDP,increased LVSP,�dp/dtmax,improved cardiac systolic and diastolic function,can slowly repair damaged myocardial tissue,improve T3,T4,TSH level in serum of rats,and inhibit ALD,BNP level in serum of rats,which play a role in the treatment of yang deficiency type of HF.3.Shenfu medicine on 1:0.5 group,aconite as monarch drug,with red ginseng compatibility,improving the dissolution rate of ginseng saponin,and reducing the contents of Aconitum alkaloids in aconite roots.To the purpose of reducing toxic and synergisting of compound Chinese medicine,and enhancing the improvement to yang deficiency type of HF effect.Therefore,its effect is superior to the rest of the Shenfu drug pair and single herb group.