Effects Of Human Umbilical Cord-derived Mesenchymal Stem Cells On A549 Cancer Cells In Hypoxia

Objective: To explore the influence about the germination of A549 between normal oxygen and hypoxia conditions under the extracorporeal circumstance with hMSCs,detect the HIF-? and Suvivin expressions in the different groups.Method: 1.Human MSCs cultures were established from umbilical cords of healthydonors by plastic adherence method,The resulted cells were further expanded and assessed for their morphology,proliferation ability by Cell Counting Kit-8 assay,immunophenotype by flow cytometry.2.To establish the indirectly co-culture between A549 cells and hMSCs,as well as cultivate separately in normal oxygen(37?,5%CO2)and hypoxia conditions(37?,5%CO2,1%O2).To observe cell migration of A549 in different group by using the Transwell chambers.To detect the survival conditions in the often oxygen and hypoxia condition separately by Annexin-V/PI Apoptosis Kit.Using the Cell Counting Kit-8 to test the diverse influence of the cell proliferation ability of A-549 under two different conditions.To detect the expression of Survivi mRNA and HIF-1? mRNA in each group of cells by qRT-PCR.Results: 1.The hMSCs were successfully isolated from human umbilical cord s' Wharton's jelly.The flow cytometry analysis demonstrated that the h UC-MSCs showed good homogeneity and expressed MSC markers CD73,CD90,CD105,but were negative for CD34,CD45,HLA-DR,CD19 and CD14 by using The flow cytometry analysis.2.After the co-culture of hMSCs and A549,with the growing of cultivating time,the proliferation of A549 is growing gradually.While after the co-cult ure of hMSCs and A549 in the normal oxygen condition,the proliferation of A549 was decreasing(p<0.05).3.Under the condition of hypoxia,,the apoptosis of A549 was decreased acc ording to the control group when co-culture with hMSCs(p<0.05).Under t he condition of normal oxygen,the co-culture of hMSCs and A549,the ap optosis of A549 was increased(p<0.05).Which showed that hMSCs could p rompt the apoptotic resistance of A549 under the circumstance of hypoxia.4.The co-culture of hMSCs and A549 in the hypoxia,in vitro migration abili ty of A549 had increased apparently(p<0.05),while the in vitro migration a bility had no significant change of the co-culture under the normal oxygen condition(p>0.05).5.Under the hypoxia conditions,the expression of HIF-1? and Suvivin of A549 in both of two groups had obvious increase than the control group.(p<0.05)Comparing with the hypoxia group the expression of HIF-1? and S uvivin of A549 was increased in the group of co-culture with hMSCs(p<0.05).Conclusion The co-culture with hMSCs and A549 cells under the hypoxia conditions could prompt the apoptosis resistance ability,proliferation ability and migration ability in vitro.HMSCs could promot Suvivin and HIF-1? expression of A549 under the hypoxia conditions.