The Expression Of Mincle In Fungal Keratitis

Objective:To investigate the expression of macrophage inducible C-type lectin(mincle)in cornea epithelium of fungal keratitis and compare with the expression of mincle in normal corneal epithelium,and analyze its difference between them.Method:A total of 54 patients with fungal keratitis at the Affiliated Hospital of Qingdao University from October 2012 to December 2013 composed experimental group,and fungus culture of all these patients were positive.Thirteen cases of healthy donor corneas were used as corneal graft,and the rest of the peripheral corneal tissues composed the control group.The cornea epithelium of the two groups were scraped.Real-time quantitative reverse transcription-polymerase chain reaction(Real-time RT-PCR)was applicated in the detection of mRNA expression of mincle receptor in corneal epithelium of the two groups.Result:Mincle expressed both in normal human corneal epithelium and FK patients.Mincle mRNA expression in FK patients were higher than that in normal cornea,and had statistically significant difference(p<0.05);fusarium-and aspergillus infected FK mincle mRNA were both higher than normal group,and had statistically significant difference(p<0.05),but the two infection groups had no statistically significant difference(p>0.05);both mild and moderate groups mincle mRNA were higher than nomal group(p<0.05),but there was no statistical difference between mild and moderate group(p>0.05);the expression of mincle during 3?7d were all higher than nomal group(p<0.05),all infected groups had no statistically significant difference(p>0.05).Conclusion:1.Mincle expressed in normal persons and fugal keratitis patients.2.The expression of mincle in fungal keratitis patients were higher than that in healthy people,and as a pattern recognition receptor mincle may participate in the innate immune response of fugal keratitis.