Cardiomyocytes Protection Of Quercetin Against Anoxia/Reoxygenation Injury Mediated By Efp

Objective:Myocardial infarction and other cardiovascular disease incidence increased year by year,the study found that the pathogenesis and myocardial ischemia reperfusion injury is closely related.Quercetin is a flavonoid plant estrogen,apple,red wine,onion,and other daily necessities were found in Green Tea.Studies have shown that^[1,2],quercetin can protect against thrombosis,and formed by the reduction of oxygen free radicals,inhibition of apoptosis,mitochondrial membrane potential play a protective effect against myocardial ischemia reperfusion injury,but the specific mechanism is still not clear.In this experiment,H₉C₂ myocardial cells,construct hypoxia/reoxygenation?A/R?injury model,to further study the mechanism of quercetin on myocardial protective effect of hypoxia reoxygenation injury of oxygen,and the relationship between estrogen and estrogen ring finger protein?Efp?.Method:H₉C₂ cardiomyocytes with good morphology were selected and randomly divided into 5 groups after routine culture:?1?Control group;?2?A/R group;?3?Quercetin+A/R group;?4?Quercetin+negative Control+A/R group;?5?Quercetin+Efp-siRNA+A/R group.The cell viability was detected by MTS;Western blotting analysis assayed the expression of Efp;the flow cytometry was used to detect the degree of cell apoptosis,the intracellular ROS and the mitochondria membrane potential;Used the euzymelinked immunosorbent assay to analyze the content of Methane Dicarboxylic Aldehyde?MDA?and glutathione peroxidase?GSH-Px?and lactate dehydrogenase?LDH?,the activities of superoxide dismutase?SOD?.Results:1.Que pretreatment of myocardial cells of the expression level of Efp,compared with the level of hypoxia reoxygenation group Efp H₉C₂ expression,the expression level of Efp and pretreatment of quercetin dose dependently up-regulated?P<0.01?;2.Que pretreatment of myocardial cells of H₉C₂ group compared with hypoxia reoxygenation group,cell survival rate increased significantly,decreased the release of LDH?P<0.01?;3.Que pretreatment of myocardial cells of H₉C₂ group compared with hypoxia reoxygenation group,decreasing intracellular MDA,SOD and GSH-Px increased levels of antioxidant?P<0.01?;4.Que pretreatment of myocardial cells of H₉C₂ group compared with hypoxia reoxygenation group,intracellular ROS content decreased significantly,increase the stability of??m,degree of apoptosis decreased;5.Que pretreatment of myocardial cells of H₉C₂group after the addition of specific Efp-siRNA adenovirus,compared with hypoxia reoxygenation group,Que in myocardial hypoxia reoxygenation injury significantly reduced the protective effect of oxygen?P<0.01?.Conclusion:Quercein against myocardial anoxia-reoxygenation injury are involved in Estrogen-responsive finger protein pathway?Efp?,and at least partly Estrogen-responsive finger protein?Efp?.