Study On Isolation And Identification,Quality Control Of Silibinin And Hypolipidemic Effects Of Tablets

Silybum marinaum (L.) Gaenrt (Chinese: Shuifeji, English: Milk thistle) is a plant of Silybum genus (Asteraceae) Asteraceae. It is the original origin from Southern Europe and North Africa.Our country as a hepatoprotective was introduced cultivation of medicinal plants in 1972.Silymarin is silymarin extract dried fruit through a flavonoid compound purified lignan obtained.The main active ingredients are four kinds of isomers,silybin?isosilybin?silychristin and silydianin.Silibinin is the most active one.Through the analysis and establishment of flavonoids content of lignan components determination of lignans(UV and HPLC)studies,on China's different regions of silymarin flavone lignans contents were measured,the source of the high content screening of silybin in silymarin extract of Chinese traditional medicine.With different concentrations of ethanol as extracting agent to extract silibinin select micro-boiling heat extraction method.When using 68%ethanol and 90%ethanol as extracting agent,the extraction capacity of silibinin is high.When the extraction temperature boil,silibinin is extracted maximum amount.Flavanolignane compounds of Silymarin is different solubilities at different concentrations of ethanol with temperature change,.to dissolve and then precipitate twice.Extraction volume is gradually increased.The effective parts further refined by ethanol obtain high-purity silibinin raw materials,and using high-performance liquid chromatography its content and related substances measured for pharmaceutical and quality research.By elemental analysis,UV,IR,NMR,MS,DSC,TQ specific rotation and powder X-ray diffraction analysis,the product is consistent with the reference structure.Silibinin isolated and purified get two monomers 1 and 2 by preparative liquid.They are analysed by NMR,MS,circular dichroism and specific rotation and determined that silibinin is two non-mixture of enantiomers,the ratio of the two stereoisomers is 1:1.Since the preparation of silibinin tablets,the establishment of dissolution methods of measurement,control the quality of the product.By increasing the solubility of silibinin improved dissolution,and predict their behavior in vivo.As an index to the dissolution of screened solid surface active agents,surface active agents,disintegrating agents,wetting agents,oil phase and fillers,silibinin tablets preparation;dissolution data have been listed according to the drug,to determine the quality of Dissolution criteria and methodology study.Using a rat model of hyperlipidemia observed on serum TC,TG,HDL-C,LDL-C,SOD,GSH-Px and MDA.Silibinin tablets of high-dose gavage eight weeks,can significantly reduce serum of hyperlipidemia rats TC,TG,LDL-C,liver MDA levels,increased serum HDL-C,SOD,GSH-Px levels.