Transcriptional Gene Expression Characteristics Of Diabetic Nephropathy And Intervention With Thalidomide

Objective:By analyzing the gene expression characteristics of diabetic nephropathy and thalidomide intervention,we were trying to analyze and summarize the gene expression characteristics at transcriptional level of diabetic nephropathy and the intervention of thalidomide on diabetic nephropathy,and to find target genes of thalidomide.Methods:The relevant datasets were searched in the GEO database of NCBI(http://www.ncbi.nlm.nih.gov/gds/)with the words “Diabetic Nephropathy” and “Thalidomide” respectively.According to the inclusion criteria and exclusion criteria,the appropriate data sets were selected.Using GEO2 R analysis methods,analysis of genes related to diabetic nephropathy and altered genes after thalidomide intervention,a gene analysis result of p<0.05 was selected.logFC<0 as gene downregulation,logFC> 0 as gene upregulation.Results:Diabetic nephropathy was used as the key words,and 9 datasets were selected.Among all these datasets,5 datasets were used as human subjects,and 4 datasets were used as mice experimental subjects.Two datasets were selected using thalidomide as a key word.Among these two datasets,one dataset was used for human and another one is used for mice.When mice were used as experimental subjects,the Ces2 g gene was used as the interference condition for diabetic nephropathy,and the log FC < 0 is statistically significant.When thalidomide was used as interference conditions,logFC > 0 is statistically significant,suggesting that the gene may be a key target gene.Conclusion:By analyzing the gene expression characteristics of diabetic nephropathy and thalidomide at the transcriptional level,thalidomide may interfere with diabetic nephropathy through intervention of Ces2 g gene expression.