Effects Of Acellular Fraction Of Advanced Epithelial Ovarian Cancer Ascites On The Biological Behaviour Of SKOV3 Cell

Objective: Ovarian cancer is the leading cause of cancer-related death in the world and has the highest mortality of any of the gynecologic cancers.The most common form of ovarian cancer is epithelial ovarian cancer(EOC).Cause the lack of syptom,most patients are FIGO IIIC-IV when diagnosed.At least one third of all cases of epithelial ovarian cancer and amost all relapse patients are associated with the production of ascites.Ovarian cancer ascites is an exudative fluid composed of a cellular fraction and acellular fraction containing rich and complicated components,which form a unique ascites-tumor microenvironment influenting biological behaviour of cancer cells.Several in vitro experiments have drawn some different conclusiones about the influences of acellular fraction of ovarian cancer on the proliferation,apoptosis,migration and invasion of overian cancer cells,which may be related to the stage,grade and histological type of cancer and also the test method.In this study,we explosed EOC cell line SKOV3 to acellular fraction of ascites from 15 patients diagnosed with advanced(FIGO ?C-?)EOC,valuated in vitro cell proliferation,apoptosis and migraton,aimed to provide basis of futher researches.Methods: Bala We collected ascites of 15 advanced EOC,centrifuged to gather the sepernatants.SKOV3 cells were grown in the medium supplemented with 5% FBS and 5% ascites for 48 h,and then proliferation,apoptosis and migraton were tested by CCK-8,flow cytometer and scratch assay respectively.Results: 1?Compared with the 5% FBS,the proliferation of all 5% ascites treated SKOV3 increased.The average proliferation ratio of 5% ascites was 1.178±0.098,which increased when compared with 5%FBS(1.053±0.125)but unstatistically.There is no significant correlation between proliferation and histological type,grade,stage,presence of neoplastic cells or chemotherapy.2?Compared with the 5% FBS,one apoptosis of 5% ascites treated SKOV3 increased,while the rest all were decreased.3?Compared with the 5% FBS,the average reduction value of nine 5% ascites treated SKOV3 increased,six of which decreased.The average values of 5%ascites were61.02±2.44,which increased when compared with 5%FBS(54.74±0.69)but unstatistically.There is no significant correlation between the average reduction value and histological type,grade,stage,presence of neoplastic cells or chemotherapy.Conclusion: 1?There is no stimulation of the effects of acellular fraction of advanced epithelial ovarian cancer ascites on the proliferation of SKOV3 cell,but both stimulation and inhibition on apoptosis and migration.2?We need more studies to make futher illunitation.