The Pharmaceutical Research Of The Citrus Medica L.Var.Sarcodactylis Granules

Compound bergamot granules are made from bergamot,bupleurum,jujube kernel,licorice.Clinically,it can adjuvant treat depression.It is the role of traditional Chinese medicine is liver qi,spleen and stomach,rather peace of mind.Depression is a chronic disease that requires long patient medication.According to the nature of the drug,prescription dose,prescription function and clinical characteristics of medication,We will be the former decoction into granules,it is easy for patients to accept,take and carry.We also systematically studied the preparation of complex bergamot particles and quality standards.According to the characteristics of the drug and physical and chemical properties,combined with the actual production,we take the oil yield,polysaccharide content and dry cream extraction rate as an indicator.In the single factor experimental method,we selected the bergamot extraction method by orthogonal experimental design method.We chose the results of bergamot by adding 10 times the amount of water,soak for 15 min,heating 4 h.According to the content of hesperidin extract of bergamot dregs,we use the response surface experimental design method.We use a decompression internal boiling extraction method.We chose the best way to extract the bergamot is the jojoba dregs by adding 85% ethanol,30 min,50% ethanol 50 ?,heating 5 min.Bupleurum,Suanzaoren and Glycyrrhiza are ethanol extraction methods.We chose the content of saikosaponin and the rate of dry cream as an indicator.We choose Bupleurum extract method based on the single factor experimental method,followed by orthogonal experimental design method.The results were as follows: Bupleurum was added to 70% ethanol,10 times,soaked for 30 min,extracted twice,and extracted for 2 h.Water extract and alcohol extract were removed,the extraction of volatile oil from the bergamot,the polysaccharide retention rate,hesperidin retention rate and the rate of dry cream as an indicator,screening the concentration of liquid pressure ratio of 1: 2.0,Centrifugal centrifugal method for centrifugal 4000 r / min,10 min;combined alcohol extract using sedimentation and removal method.Alcohol extract after the recovery of ethanol to non-alcoholic,mixed with water extract,concentrated under reduced pressure to the relative density of 1.3 ~ 1.35(60 ?)of the cream.Dried under reduced pressure into dry paste(80 ?),playing powder,sifted.The results show that the pigment is 65%,lactose 10% and dextrin 25% by wet granulation,and the volatile oil is used as the index.To 90% ethanol dissolved into the granules,sub-equipment,that is compound bergamot granules.Quality standards,the bergamot,Bupleurum and other four herbs were thin layer identification,high degree of separation,negative without interference;compound bergamot granules determination by HPLC determination of hesperidin and saikosaponin a content,and the method Study,the initial preparation of this product per gram containing hesperidin amount of not less than 1.04 mg,per gram of saikosaponin a amount of not less than 2.35 mg.In the stability test,the product for 6 months of accelerated stability test and 12 months of long-term stability test,the results of the inspection indicators are in line with the provisions and the product are stability and controllable within a year.