Study On The Correlation Between Chronic Endometritis And Recurrent Spontaneous Abortion

Objective?to investigate the role of CD138 in the diagnosis of chronic endometritis(CE);?to investigate the correlationbeet between recurrent spontaneous abortion(RSA)and chronic endometritis(CE).Methods1.Case informationFrom October 2015 to October 2017,selected 80 patients with recurrent spontaneous abortion who asked for induced abortion operation in the outpatient department of the First Hospital of Shanxi Medical University,who were aged 24 ~ 35 years old,the average(29.18±3.29)Age of age,menopause time of 53 ~ 80 days,abortion number 2 ~ 5 times,the average(2.31 + 0.47);According to the following conditions: menopause time<12 weeks,B ultrasonography showed no embryo or pulse tube pulsation,indicating That the embryo stopped developing and had no symptoms of threatened abortion;All patients need to exclude the embryo chromosome abnormality,chromacy abnormalities,couples chromosomal abnormalities,genital malformations,endocrine abnormalities,infection,autoantibody positive,at the early pregnancy use drug history,radiation Exposure history,husband sperm abnormalities.In the same period,50 cases of healthy pregnant women Induced abortion in our hospital were randomly selected as the control.According to the following conditions: age 24 ~ 35 years old,physical health,menstruation rule,no natural abortion,stillbirth history,B ultrasonography proposals that the embryo development is normal.2.Experimental methodsCollection of recurrent abortion patients and healthy pregnant women abortion of endometrium after,immediately wash with physical saline,remove residual blood,10% formalin fixed for 24 hours,dehydration,paraffin embedding,collection of patients with recurrent spontaneous aborition embryo down at the same time the third party inspection agency villi chromosome examination,excluding miscarriage cases caused by embryonic chromosomal abnormalities,80 cases of recurrent spontaneous abortion patients and 50 cases of early pregnant women were eligible for endometrial resection.The wax blocks were serially sectioned at 3 to 4 mm.Each specimen was treatment to HE staining and CD138 immunohistochemical staining.The plasma cells were looked for under a microscope and the number of positive cells was viewed under a digital pathological scanning system.Pathological results were determined by two experienced pathologists independently looking for plasma cells on the same section and confirmed.Comparison of the detection rates of CE between the two experimental methods and discusse the differences in the detection rates of CE in recurrent spontaneous abortion group and normal group.3.Statistical methodsSPSS 20.0 software was used for statistical analysis.The normal measurement data was represented by “x±s”.The number of count data use cases(n)was expressed.The ratio between groups(%)was compared by ?2 test.P<0.05 was considered statistically significant.Result1.in the recurrent abortion group,The positive rate(16%)of the CD138 immunohistochemistry staining and the positive rate(8.7%)of the HE staining were significantly different(?2=4.17,P<0.05).2.The positive rate of CD138(16%)in the recurrent spontaneous abortion group was significantly significant compared with the normal control group(4%)(?2=4.52,P<0.05).conclusion1.CD138 immunohistochemical staining have the great value for the diagnosis of chronic endometritis;2.Chronic endometritis has a certain correlation with recurrent miscarriage.