Comparative Study On The Chemical Components And The Sedative Effect Of Different Combinations Of Suanzaoren And Fuling

Objective In Suanzaoren and Fuling medicine as the research object,the different processed Suanzaoren and Fuling in different proportions,determining the content of different combinations' Jujuboside A,spinosin,total saponins,total flavonoids and polysaccharide and efficacy in the treatment of insomnia research.Choose a optimal combination from the research's result of established quality standards,in order to provide a scientific basis for further development and utilization of this couplet medicines.Method With Suanzaoren's different processed products-raw,fried and fried half and half,and Fuling by 1:0,1:1,1:2,1:3,3:1,2:1,0:1 ratio of compatibility,a total of 19 different combinations.Using HPLC and UV spectrophotometric method for the determination of the content of Suanzaoren saponin A,spinosin,saponins,flavonoids,polysaccharides and compare their differences;at the same time with Kunming mice and SD rats by three kinds of collaborative experiment experimental method,influence on the spontaneous activity of influence on suspension tail reaction and pentobarbital sodium on Seed and poria drugs on the sedative and hypnotic effects of different combinations,select the better combinations from and to establish its quality standard.Result 1 chemical part:?Fuling to jujubosides A and spinosin,total saponins,flavonoids,polysaccharides dissolution effect trend to increase the compatibility of Suanzaoren processed and non processed products also have a certain influence on the composition of the dissolution?Shengsuanzaoren and Fuling Ratio(Raw group)3:1's Jujuboside A,spinosin,total saponin;Raw group 2:1's total flavonoids,polysaccharide content were the highest in the combination of19.?Raw group 3:1's Jujuboside A,spinosin,total saponin and Raw group 2:1's total flavonoids,polysaccharide are the highest in the Raw group.after processing zaoren and Fuling ratio(Fried group)1:1's Jujuboside A,spinosin,total flavonoids,total saponins and Fried group 3:1's polysaccharide are the highest in the Fried group.before and after processing zaoren half and half and Fuling ratio(Half group)3:1's Jujuboside A,spinosin,polysaccharide?Half group2:1's total saponin and Half group1:2's total flavonoids are the highest in the Half group.2 Efficacy Test shows:? The combination of the 19,Raw group 1:0,1:1,1:2,2:1,1:3 could significantly reduce in mice tail suspension to stationary latency(P<0.05 or P<0.01),Fried group 1:0,1:1,3:1 could significantly reduce the number of standing mice(P<0.05),Half group 1:2,3:1 group could significantly reduce the number of spontaneous activity of mice(P<0.01).?Further experiments to select Raw group 1:2,2:1;Fried group 1:1,3:1;Half group 1:2,3:1 a total of six groups of pharmacodynamics experiment.Raw group 1:2 2:1 were significantly reduce in the tail suspension to a static incubation period(P<0.05),Fried group1:1,3:1could significantly reduce the number of standing mice(P<0.05),Half group 1:2,3:1 group could significantly reduce the frequency of spontaneous activity of mice(P<0.05).?Choose again Half group 1:2,3:1 two groups of rats pharmacodynamic experiment.The results showed that the two groups were significantly reduced in mice or rats independent activity frequency(P<0.05 or P<0.01);Half group 1:2 can significantly reduce the number of rats standing and significantly shorten the latent period in conjunction with sodium pentobarbital induced sleep(P<0.05).The final choice is Half group1:2.3Quality standard:The initial quality standard 1:2(3g)shall not exceed 15% for moisture provisionally,total ash not more than 2%,the extract is not less than 10%,HPLC measurement of jujubosides A shall not be less than 0.03%,spinosin shall not be less than 0.08%,not less than 0.66% of total polysaccharides was determined by UV spectrophotometry.Conclusion?The content changes of chemical constituents in Fuling with Suanzaoren in 19 groups showed exiting regularity,Suanzaoren with Fuling have an important influence on the dissolution of chemical components may be the common effect of multiple components.?Suanzaoren different combination of Fuling have effect on treatment of insomnia,among the combinations Half group1:2 were better sedative and hypnotic effects(P<0.05).?The initial quality standard protocoled with developed functional food of Fuling and Suanzaoren.