| Objective:Observe the effect of Qiangjing Tablet on TGF-?1/Smads signaling pathway in patients with asthenospermia,and explore the possible mechanism of its treatment for asthenospermia,provide possible ideas for further study of the drug,and study the treatment mechanism of asthenospermia The theoretical basis.Methods:From January 2017 to December 2017,72 cases of infertility patients who were diagnosed as having kidney deficiency and blood stasis asthenospermia were selected from infertility clinics at the male outpatient department of the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,and the woman had no abnormal reproductive function.Randomly divided into experimental and control groups,36 patients in each group,and the experimental group received oral strong fine tablets(provided by the Chinese medicine processing room of the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine),4 tablets each time,3 times a day,and the control group was given oral Wuzi Yanzong Pills(From the Chengdu University of Traditional Chinese Medicine Affiliated Hospital pharmacy),each time 6g,2 times a day,were treated for a period of 4 weeks,comparing the percentage of sperm forward movement before and after treatment in two groups of patients(PR),seminal plasma TGF-?1 And the expression of protein Smads2,Smads7,TCM syndrome score changes.Results:1.The average PR of the experimental group before treatment was 18.84±7.44%,and the average PR after treatment was 26.77±10.68%.The difference between before and after treatment was statistically significant(P<0.05).The average PR before treatment was 16.44±9.07% in the control group after treatment.The average PR was 21.35±8.68%,and the difference was statistically significant(P<0.05).The difference between the two groups after treatment was statistically significant(P<0.05).2.The average TGF-?1 before treatment in the experimental group was 210.62±7.53 pg/mL,and the mean TGF-?1 after treatment was 198.35±6.68 pg/mL.The difference was statistically significant before and after treatment(P<0.05);-?1 was 186.75±4.77 pg/mL,and mean TGF-?1 was 177.21±7.88 pg/mL after treatment.The difference was statistically significant(P<0.05).There was no significant difference between the two groups after treatment(P>0.05).3.The average Smads2 concentration in the experimental group before treatment was 2.51±0.25ng/mL,and the Smads2 concentration after treatment was 4.83±0.96ng/mL.The difference was statistically significant before and after treatment(P<0.05).The average Smads2 concentration in the control group before treatment was 2.41±0.98.The ng/mL,Smads2 concentration after treatment was 3.72±1.01 ng/mL,and the difference was statistically significant(P<0.05).There was no significant difference between the two groups after treatment(P>0.05).4.The average Smads7 in the experimental group was 10.60±1.12 ng/mL before treatment,and the mean Smads7 was 3.26±1.38 ng/mL after treatment.The difference was statistically significant before and after treatment(P<0.05).The average Smads7 before treatment was 12.50±0.69 in the control group.Ng/mL,mean Smads7 after treatment was 5.75±1.77 ng/mL,with significant difference before and after treatment(P<0.05).There was no significant difference between the two groups after treatment(P>0.05).5.According to statistical analysis of TCM syndrome scores,after treatment,the experimental group had 4 clinical controls,13 markedly effective,11 effective,8 ineffective,and 77.78% total effective rate.The control group had 3 clinical controls and 12 markedly effective.9 cases were effective,12 cases were ineffective,and the total effective rate was 66.67%.Comparing the total effective rate between the two groups,P=0.012,P<0.05,statistically significant.Conclusion:1.Compared with Wuzi Yanzong Pills,Qiangjing Tablets can significantly improve the sperm forward mobility in patients with weak sperm;2.Qiangjing Tablets and Wuzi Yanzong Pills can activate the expression of Smads2 in seminal plasma of patients with asthenospermia,inhibit the expression of TGF-?1 and Smads7,activate the TGF-?1/Smads signaling pathway,and achieve intracellular and extracellular signal transduction.3.Compared with Wuzi Yanzong Pills,Qiangjingjing Tablet can significantly improve the score of TCM syndromes in patients with kidney deficiency and blood stasis asthenozoospermia. |
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