Emerging Role Of Growth Differentiation Factor 11 In Adipogenesis And Lipid Metabolism

Objective:Growth differentiation factor(GDF11),also called as bone morphogenetic protein11(BMP11)belongs to the transforming growth factor-?(TGF-?)superfamily,plays an important role in embryonic development and anti-aging.But its role in adipose-derived stem cells and fat metabolism is unknown.In this study,GDF11 protein was purified by gene engineering and its effect on adipose-derived stem cells and fat metabolism was examined.Methods:1.The pc DNA3.1~+-KSH-DHFR-GDF11 plasmid was constructed by genetic engineering technology.2.The plasmid was transformed into CHO cells to construct the GDF11eukaryotic expression system,and the GDF11 was purified through Ni-chelating affinity chromatography.3.The effect of GDF11 on the proliferation of adipose derived stem cells was detected by CCK8 method.The effect of GDF11 on adipogenic differentiation of adipose derived stem cells was detected by oil red O staining and q-PCR.4.Using oil red O staining,triglyceride detection,glycerol detection,etc.to explore the effect of GDF11 on fat metabolism.Results:1.The pcDNA3.1~+-KSH-DHFR-GDF11 plasmid were successfully constructed by colony PCR and double enzyme digestion.2.The expression of GDF11 protein was detected by Coomassie blue staining.3.GDF11 can promote the proliferation of adipose-derived stem cells,and inhibit adipose stem cell adipogenic differentiation.4.GDF11 inhibits fat anabolism and promotes fat catabolism in adipocytes.Conclusion:1.The GDF11 protein was successfully expressed and purified.2.GDF11 promotes the proliferation of adipose stem cells.3.GDF11 inhibits adipogenic differentiation of adipose stem cells.4.GDF11 inhibits fat anabolism and promotes fat catabolism in adipocytes,can reduce lipid accumulation in adipocytes.