| According to the latest statistics,in 2017,there were 14.09 million new cases of cancer worldwide and 8.8 million deaths.In 2017,there were 3.68 million new cancer cases and more than 2.16 million deaths in China.Compared with previous years,the incidence and mortality have risen significantly.The situation that cannot be optimistic brings enormous burdens to society,countries,families and patients.However,in cancer,lung cancer is at the top of morbidity and mortality for many years,because of its high metastasis rate and drug resistance,causing patients with short postoperative survival and poor prognosis.In-depth studies of traditional Chinese medicine in recent years have found that traditional Chinese medicine has a wider range of anti-cancer effects than traditional chemical drugs that are prone to highly resistant.Cel can induce apoptosis of tumor Cells and inhibit the metastasis of tumor Cells,and is highly effective and low in toxicity.According to related reports,TGF-?1 is also closely related to the epithelial mesenchymal transition,metastasis,invasion,and vasculogenic mimicryof various tumor Cells.KLF8 is closely related to the migration,invasion,vasculogenic mimicryand epithelial-mesenchymal transition of various tumor Cells,and the expression level of KLF8 in non-small Cell lung cancer is significantly higher than that in normal lung Cells.KLF8 may be used for vasculogenic mimicryin non-small Cell lung cancer.There is also a lack of research on the mechanism of action with transformation.Therefore,the A549 Cell was the experimental material and Cel was the drug that I uesd to explore the effect of TGF-?1 on the vasculogenic mimicryand metastasis of non-small Cell lung cancer A549 Cells and the effects of Cel on A549 Cell blood vessels.Generation and transfer effects.1.This paper mainly carried out the following work:(1)The effect of Cel on the activity of A549 Cells was detected by MTT assay.The effect of TGF-?1 on the activity of A549 Cells was detected by MTT assay.MTT assay was used to detect the effect of TGF-?1 and Cel on the activity of A549 Cells.(2)Morphological observation The effect of TGF-?1 on the morphology of A549 was observed.Morphological observation experiment was conducted to observe the effect of TGF-?1 and Cel on the morphology of A549.(3)Tubule formation assay Tripterygium on A549 Cells in vitro tubular capacity.Tubulogenesis assay was used to detect the effects of TGF-?1 and Cel on the in vitro tubulogenesisof A549 Cells.(4)scratch test Triptolide A549 migration.Scratch assay was used to detect the effect of TGF-?1 and Cel on A549 migration.(5)Cell adhesion assay to detect the influence of Cel on the adhesion of A549 Cells.Cell adhesion assay was used to detect the effect of TGF-?1 and Cel on the adhesion of A549 Cells.(6)The influence of Cel on the invasiveness of A549 Cells was detected by Transwell assay.The effects of TGF-?1 and Cel on the invasiveness of A549 Cells were detected by Transwell assay.(7)The effect of TGF-?1 and Cel on the expression of KLF8 and VEGF in A549 Cells was detected by Western Blot.2.Through experiments we get the following findings:(1)MTT assay showed that Cel reduced the activity of A549 Cells in a time-and dose-dependent manner.When the concentration of TGF-?1 was between 1ng / m L and 10 ng / mL,A549 Cells were treated with 5 ng / mL of TGF-?1 for 5 days,respectively.When A549 Cells were treated with different concentrations of Cel,the A549 Cell activity decreased with the increase of Cel concentration.(2)Morphological observation showed that A549 Cells treated with 5ng / mL and10ng/mL of TGF-?1 exhibited typical epithelial-mesenchymal transitional morphology.Cel inhibited TGF-?1-induced epithelial-mesenchymal transition in A549 Cells.(3)tubule formation experiment found that Cel inhibited A549 Cell tubule formation,with the increase of Cel concentration,the number of A549 tubules reduced,the official cavity more and more broken.Cel inhibited TGF-?1-induced tubular formation in A549 Cells.(4)scratch repair experiments found: Cel inhibits A549 Cell migration,the greater the concentration,the stronger the inhibition.TGF-?1 can promote the migration of A549 Cells.Cel can inhibit TGF-?1 to promote the migration of A549 Cells.(5)Cell adhesion experiments showed: Cel can effectively reduce the A549 Cell adhesion rate,the higher the drug concentration,the lower the adhesion rate of A549 Cells.TGF-?1 can promote the adhesion of A549 Cells.Cel can inhibit the adhesion of TGF-?1 to A549 Cells.(6)Transwell experiments showed that Cel inhibited the invasion ability of A549 Cells,and the higher the concentration,the less A549 entered the lower chamber.TGF-?1 can promote the invasion of A549 Cells.Cel can inhibit TGF-?1 to promote the invasion of A549 Cells.(7)Western Blot experiments showed that: TGF-?1 can promote the expression of KLF8 protein and VEGF protein in A549 Cells,while Cel inhibits the up-regulation of KLF8 and VEGF protein by TGF-?1.In summary,TGF-?1 can up-regulate the expression of KLF8 and VEGF in A549 Cells and further promote the vasculogenic mimicryof A549 Cells.Cel significantly down-regulated the expression of KLF8 protein,thereby inhibiting the vasculogenic mimicryand metastasis of TGF-?1-stimulated A549 Cells.In this paper,we investigated the effects of Cel and TGF-?1 on the vasculogenic mimicry and metastasis of A549 Cells,which provided a theoretical basis for further understanding the anti-tumor mechanism of Cel. |
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