Human Adipose-derived Mesenchymal Stem Cells Relieve Acute Hepatocellular Failure By Paracrine Inhibition Of Oxidative Stress

Objective:To explore the possible mechanisms of Human adipose-derived mesenchymal stem cells relieve acute hepatocellular failure.Methods:1?60 rats were randomly divided into normal control group,model group and stem cell transplantation group,20 rats in each group.We established acute liver failure model by intraperitoneal injection of D-galactosamine.The rats in the control group were injected with the same amount of saline.After the model was established,We given model group and stem cell transplantation group normal saline or human ADMSCs?3.0×10 ⁶?via the tail vein.The levels of serum alanine aminotransferase?ALT?,aspartate aminotransferase?AST?and oxidative stress of MDA and SOD in rats were measured at 1,3,7 days after transplantation.The pathological changes of liver were observed by HE staining.2?The normal liver L02 cells were randomly divided into normal control group,model group and experimental group.Model group and experimental group were treated with H₂O₂ to construct hepatocyte failure environment.The experimental group was treated with ADSCs conditioned medium.Then,The level of the MDA would be detected by reagent kit,and the survival of the cells was detected by CCK-8.Results:The levels of ALT,AST and MDA in the model group and the treatment group were significantly higher than those in the control group?P<0.05?.Compared with the model group,the levels of serum ALT?AST and the expression of MDA of the treatment group were significantly decreased,and the expression of SOD higher than control group?P<0.05?.The results of hematoxylin-eosin staining indicated a significant improvement in liver degeneration and necrosis.The results of oxidative stress in the hepatocellular model group were significantly higher than those in the control group and the experimental group?P<0.05?.We noticed that the results of the experimental group compared with the control group are similar?P>0.05?,which proved that oxidative stress response was effectively controlled and the treatment was effective.The results of CCK-8showed that the cell viability in the model group and the experimental group was significantly lower than that in the control group?P<0.05?,but the cell viability was higher in the experimental group than in the model group?P<0.05?.Conclusion:Oxidative stress response was involved in the development and progression of acute hepatocellular failure.Human adipose-derived mesenchymal stem cells relieve acute hepatocellular failure by paracrine inhibition of oxidative stress.