Antagonistic Effect Of Perfluorocarbon Emulsion On Vasomotor Activity Factor Of AS Rats

Objective: At present,the inflammatory response is thought to run through the whole process of initiation,formation and development of atherosclerosis(AS),and its first and earliest link is endothelial dysfunction.Therefore,the protection of endothelial cell function and anti-inflammation treatment is particularly important in the prevention and treatment of AS.Perfluorocarbon(PFC),as the ideal carrier of oxygen,has been proved to have certain nonspecific anti-inflammatory and cellular protective effects in recent years.In this experiment,the perfluorocarbon emulsion(PFCE)was prepared by using a high shear emulsifier.The rats in the atherosclerotic model were treated by intravenous injection of PFCE to observe the changes of endothelial function and anti-inflammatory action.Method: Fifty Wistar males were randomly divided into 5 groups: blank control group,model group,PFCE low,medium and high dose group,10 rats in each group.In the control group,the experimental rats were induced by vitamin D3 plus high fat diet,and the treatment group was treated with different doses of drugs at the same time.After 12 weeks,the levels of serum nitric oxide(NO),endothelin-1(ET-1),thromboxane A2(TXA2),6-keto-prostaglandin(6-K-PG),oxidized low density lipoprotein(ox-LDL)and monocyte chemoattractant protein(MCP-1),in each group were measured and the pathological changes of the aorta were observed by HE staining.Result: The content of NO and 6-K-PG in model group was significantly decreased(P<0.05),and the content of ET-1,TXA2,ox-LDL,MCP-1was significantly increased,and the ratio of NO/ET-1 and TXA2/6-K-PG were significantly different from the control group(P<0.05).The levels of NO and 6-K-PG in the high and middle dose groups of PFCE were significantly higher than those in the model group,and the content of ET-1,TXA2,ox-LDL,MCP-1 was significantly decreased and the ratio of NO/ET-1 and TXA2/6-K-PG was improved(P<0.05).There was no significant difference in the serum levels between the low dose group and the model group(P>0.05).The levels of NO and 6-K-PG in the PFCE high dose group and the low dose group were significantly higher than those in the low dose group,and the content of ET-1,TXA2,ox-LDL,MCP-1.And the difference was not significant in the middle dose group(P<0.05).The PFCE high and middle dose group reduced the morphological changes of the aorta in the AS group compared with the PFCE low dose group(P>0.05).Conclusions: Medium dose above perfluorocarbon emulsion may protect endothelial function through regulating the vasculature AS rats vasomotor activity factor and suppressing inflammatory factor,play a role in preventing the occurrence and development of AS further.