| Background and Objective: Bladder cancer is a malignant tumor that occurs in the bladder mucosa.It is one of the most common malignant tumors of the urinary system.Worldwide,the incidence of bladder cancer is 8th among males and 25 th in females.Zinc ion transporters are significant for normal cellular functions by firmly regulating concentration of zinc ions in somatic cells.Incidence increases with age,with a high age of 50 to 70 years.The incidence of males and females is 3:1.This study is a population-based case-control study that fully considered the effects of possible confounding factors and analyzed the association between the zinc transporter gene ZIP11rs11077654 polymorphism with bladder cancer risk in a Chinese Han Population.Many studies have shown proof of relationship between zinc ions imbalance,Zinc transporters dysfunction and the development of urological cancers.Four variants(rs11871756,rs11077654,rs9913017,rs4969054)within Zinc transporters ZIP11 were significantly associated with bladder cancer risk.It is expected to make a correct and objective evaluation of the relationship between the polymorphism of this gene and bladder cancer.Methods: The subjects from the Chinese Han Population were divided into bladder cancer group(139 cases)and healthy control group(168 cases).The diagnosis of bladder cancer patients was confirmed with transitional cell carcinoma of bladder histologically through pathological examinations.In the healthy control group,physical examination,imaging examination and laboratory examination excluded infection,inflammation,tumor,autoimmune diseases,gastrointestinal tract,liver and endocrine diseases.Collecting demographic data through face-to-face interviews,all individuals signed informed consent and used EDTA anticoagulated venous blood(5ml)for all subjects.All specimens excluded hemolysis and lipemia.DNA was extracted from the blood samples using a finished product kit.The ZIP11 rs11077654 loci was analyzed by Polymerase Chain Reaction(PCR).The DNA was sequenced using MassARRAY 4.0 analytical mass spectrometer.The results obtained were analyzed by TYPER 4.0 software.The data obtained were analyzed by SPSS 17.0 software.Statistical analysis was performed with P<0.05 as a statistically significant threshold.Values and 95% confidence interval(95%CI)showed relative risk to compare the differences between the bladder cancer group and healthy population,and analyze the correlation with bladder cancer.Results: There were three kinds of genotypes in ZIP11 gene rs11077654 loci(CC,CA and AA).Our analysis presented that the CA genotype was not associated with bladder cancer risk when the CC genotype served as reference(OR,1.355;95% CI,0.750-2.451;p=0.31?3).The AA genotype was not associated with bladder cancer risk(OR=1.169;95% CI=0.256-5.335;p=0.317).Additionally,considering the association of rs11077654 C allele with bladder cancer and the relatively small number of CA genotype,compared with CC genotype,we combined CA and AA genotypes as a dominant genetic model.There was no significant association with bladder cancer risk as well in the combined group(OR,1.333;95% CI,0.758-2.343;p=0.317).There was no significant association observed between the CA/AA genotypes and low(OR,0.900;95%CI,0.411-1.973;p = 0.793)or high-grade bladder cancer(OR,0.675;95% CI,0.349-1.308;p = 0.243),and nor between the CA/AA genotypes and NMIBC(OR,0.636;95% CI,0.334-1.213;p = 0.167)or MIBC(OR,1.100;95% CI,0.478–2.531;p = 0.823).Conclusion: ZIP11 gene shows different variation spectrum between different areas.The rs11077654 of ZIP11 gene would not be associated with bladder cancer in Chinese Han Population. |
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