The Expression And Significance Of HADH And SRC In Ovarian Cancer

Background and ObjectiveOvarian cancer is one of the deadliest tumors in gynecologic malignancies,which is not related to its obvious clinical symptoms in early stage.Current evidence has been used to find early and effective screening mechanisms before the onset of clinical symptoms.Therefore,biomarkers are the key to predict disease progression and risk,which can improve the diagnostic accuracy of ovarian cancer and ensure early diagnosis.Recent studies have revealed a number of biomarkers,such as serum protein markers,CA-125,HE4,mesothelin,which have high sensitivity and specificity in the early stages of cancer.Therefore,our hypothesis is that biomarker panel panels will be more effective in improving the prognosis of patients with high sensitivity and specificity of survival.Early detection of ovarian cancer and molecular mechanisms of ovarian cancer treatment is an urgent need.Hydroxyacyl-CoA dehydrogenase(HADH)is a member of the3-hydroxyacyl-CoA dehydrogenase gene family.HADH-encoded proteins function in the mitochondrial matrix to catalyze the oxidation of linear 3-hydroxyacyl-CoA as part of the?-oxidation pathway.Among them,medium chain fatty acid has the highest enzyme activity.The mutation in this gene led to a familial hyperinsulinemic hypoglycemia.Src Non-receptor tyrosine kinase(Src)This gene is highly similar to the r-src gene.This proto-oncogene plays an essential role in the process of embryonic developments and cell growth.The protein producted by this gene is an important protein kinase of whose activity may be inhibited by c-SRC kinase phosphorylation.This gene mutation could be involved in the malignant development of colon cancer.At present,the study of these two genes mainly in other tumors,and ovarian cancer rarely reported,so this article by detecting HADH and SRC in ovarian cancer patients and normal tissue differences between the analysis of the content and ovarian Cancer prognosis,to explore its potential as a molecular marker of prognosis and treatment of ovarian cancer.MethodUsing bioinformatics methods to identify differential genes.The ovarian cancer gene chip data was downloaded and compiled from the GEO(http://www.ncbi.nlm.nih.gov/geo)database.Differential genes were obtained by differentially expressing genes for data analysis.Gene Ontology analysis and signal pathway analysis(Kyoto Encyclopedia of Genes and Genomes)were performed.Finally,Cytoscape was used to construct the protein interaction network(PPI)for all the differentially encoded proteins.We collected from June 2013 to June 2015,People's Hospital of Qingzhou of gynecological 72 cases of ovarian cancer patients with normal tissue(tumor> 5cm)and tumor tissues.The RNA of ovarian cancer tissue and normal tissuewere extracted byTrizol method.Using a micro-spectrophotometer to detect the purity and concentration of extracted RNA,take the appropriate purity(1.80-2.0)between the RNA reserve for the next experiment,those who do not meet the requirements are re-extracted until the purity requirements.The sequences of HADH and SRC in Gene database were used to design HADH and SRC using the primer design software of Stanford University.GAPDH was used as a control.Then reverse transcription Real-time PCR was used to detect the expression of HADH and SRC in tumor tissues and normal tissues of ovarian cancer patients by real-time quantitative polymerase chain reaction.According to the clinical information of patients,the expression levels of HADH and SRC were analyzed by SPSS19.0,The relationship between features.ResultsThe expression of HADH and SRC in ovarian cancer tissues was obviously higher than the expression in normal tissues,and the differences were statistically significant(P<0.01).There was no correlation between the expression level of HADH and SRC in age,tumor size and pathologic type(P> 0.05),but with the degree of lymph node metastasis,tumor differentiation and TNM stage(P <0.05).Kaplan-Meier survival curve analysis showed that the 3-year progression-free survival of HADH and SRC overexpression groups were significantly decreased compared with the corresponding low expression group(P <0.05).The areas under the ROC curve of HADH and SRC were 0.753 and0.841,respectively(P <0.05),indicating that these two genes have high diagnostic value,high sensitivity and specificity,consistency test the result is better.ConclusionsThe expression of HADH and SRC in ovarian cancer tissues is higher than that in normal tissues.Ovarian cancer with high expression of HADH and SRC is often staged late,and the prognosis is poor.HADH and SRC are closely related to the occurrence and development of ovarian cancer,which can serve as a new tumor molecular marker for the early diagnosis and treatment of ovarian cancer.