| Objective To investigate the expression of ALPL(alkaline phosphatase,liver/bone/kidney)in human ovarian cancer cell lines and tissues,and its function or mechanism in cell proliferation and tumorigenesis in SKOV3 cell was assessed.Methods 1.ALPL mRNA and protein level were measured by qRT-PCR and Western Blot in ovarian cancer cell lines or tissues and compared with that of normal ovarian tissues.2.Forced ALPL expression was induced in SKOV3 cell by interfered lentivirus,and cell proliferation and cell cycle were assessed by MTT assay,plate colony formation assay and flow cytometry.3.Tumorigenesis was evaluated through xenograft growth in nude mice.3Three key regulatory molecules in Wnt / ?-catenin signaling were detected by Western Blot.Results Significantly lower expression of ALPL was found in all ovarian cancer cell lines or tissues studied,compared with that in normal ovarian tissues.ALPL Over-expression was observed to decrease the cell proliferation and clone formation ratein SKOV3 cell,to block the transition of G1 phase to S phase in vitro.However,no effect on cellapoptosis was observed.Moreover,subcutaneous xenografts tumors from ALPL-SKOV3 cells present with smaller tumor volume and lower weight.Activity of Wnt signaling was significantly attenuated.Conclusion ALPL might act as a tumor suppressor in ovarian cancer through inhibiting cell proliferation and blocking cell cycle transition from G1 to S phase,probably through suppressing canonical Wnt / ?-catenin signaling. |
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