The Relationship Among HIF-1?,TNF-? And IL-6 The Change Of The Biological Behavior Of Human Dermal Microvacular Endothelial Cells After Silencing Gene HIF-1? Under Hypoxia

Objective : This work is aimed to study the relationship among hypoxia inducible factor-1?(HIF-1?),tumor necrosis factor-?(TNF-?)and interleukin-6(IL-6)and observe the change of the biological behavior of human dermal microvascular endothelial cells(HDMECs)after silencing HIF-1? gene under hypoxia.Methods : A three-gas incubator was used to cultured the HDMECs,which were grouped by the time and oxygen concentration to determine the optimum culture time and hypoxia concentration.Meanwhile,adenovirus targeting HIF-1? was constructed and infected HDMECs.The HDMECs were divided into control group,non-HIF-1?-siRNA group and HIF-1?-siRNA group.RT-PCR and Western blot were then used to detect the mRNA and protein levels of HIF-1?,TNF-? and IL-6,respectively.The cell migration ability was detected by Transwell,and apoptosis and cycle changes were observed by flowcytometry.Results:Under hypoxia,the gene expressions of HIF-1?,TNF-? and IL-6 increased with the increase of hypoxia time,and increased with the decrease of oxygen concentration.Compared with the control group,the cell migration ability(P<0.01)and the cycle(P<0.01)of the HIF-1?-siRNA group were significantly inhibited,and the apoptosis was significantly increased(P<0.01).Compared with the control group,the mRNA and protein levels of TNF-? and IL-6 genes in HIF-1?-siRNA group were decreased(P<0.01).Conclusion: under hypoxia,the gene expressions of HIF-1?,TNF-?and IL-6 were in time dependent and oxygen concentration decreasing dependent manner.The expression of HIF-1? in HDMECs was positively related to inflammatory factors TNF-? and IL-6.Silencing HIF-1? can obviously inhibit HDMECs proliferation,migration and promote apoptosis.