| Objective:1 To investigate the expression of type II glutamate vesicle tra nsporter(VGLUT2)in spinal cord dorsal root ganglia(DRG)of ort hotopically transplanted human ovarian cancer.2 Effect of endothelin on VGLUT2 in DRG of mice.Methods:1 Fifty five-week-old unmatched female Kunming mice were se lected to be 25 g or so.The mice were divided into 5 groups(n=10)by random number table method.They were sham operation gro up,model control group,and model ET-1 group,model BQ123 gr oup,model BQ788 group.The model control group used the dorsal approach to implant the mouse ascites tumor S180 tumor cells in situ in the left ovary.Drug interventions were performed on the 8th,10 th,12th,and 14 th days after the establishment of the model in the drug group.The endothelin receptor agonist ET-1 was used to i ntervene in the model ET-1 group,and the endothelin A receptor a ntagonist BQ123 was administered intrathecally into the BQ123 gro up.The Endothelins B Receptor(ETBR)antagonist BQ788 was ad ministered to the model BQ788 group.2 Behavioral tests:Withdrawal mechanical threshold and paw wi thdrawal thermal latency were measured before he establishment of the model.And mice were tested on the 7th,9th,11 th,and 13 th da ys after the establishment of the model.3 On the 14 th day after model establishment,mice were killed and lumbar spinal dorsal root ganglion was taken.The average optical density(MOD)of different groups of VGLUT2 was detected byimmunohistochemistry.The difference of VGLUT2 protein expression in each group was determined by Western blotting.Results:1.Behavioral experiments: The mechanical and thermal pain thresholds of the mice in the model group were lower than those in the sham group.After the model ET group was self-administered,the mechanical and thermal pain thresholds were significantly decreased compared with the model control group(P<0.05).After given BQ123,the mechanical and thermal pain thresholds of mice were increased compared with the model control group(P<0.05),but did not return to normal levels(P<0.05),and compared with ET-1 group,threshold was increased(P<0.05),and the pain threshold also increased compared with the BQ788group(P<0.05).The mechanical and thermal pain thresholds of mice injected with BQ788 were also significantly increased(P<0.05)compared with the model group(P<0.05),but lower than the sham group(P<0.05).And also significantly higher than those of the ET-1group(P<0.05).2.Immunohistochemistry: The VGLUT2 MOD of the model control group was significantly greater than that of the sham group(P<0.05).The VGLUT2 of DRG in model ET group was higher than those of model control group(P<0.05).The MOD of VGLUT2 in DRG of BQ123 group was significantly lower than that of the model control group(P<0.05),and lighter than that of model ET-1 group(P<0.05)and model BQ788(P<0.05).The VGLUT2 MOD of Model 788 was not significantly different from the model control group(P>0.05),and was lighter than the model ET-1 group(P<0.05).3.Western blot: The expression of VGLUT2 in the model control group was significantly higher than that in the sham group(P<0.05).The protein content of VGLUT2 in model ET intervention group was significantly higher than that in model group(P<0.05).The expression of VGLUT2 in the model BQ123 group was less than that in the modelcontrol group(P<0.05).Compared with the model ET-1 group,the expression level of VGLUT2 was decreased(P<0.05).Compared with the model BQ788 group,the expression level was also lower(P<0.05).The expression of VGLUT2 in the model BQ788 intervention group was not significantly different from that in the model group(P>0.05),and was lower than that in the model ET-1 group(P<0.05).Conclusions:1.VGLUT2 was different between the sham group and the model group,and the model control group was significantly more.2.The expression of VGLUT2 in the ET-1 group was higher than that in the BQ123 and BQ788 groups.The VGLUT2 in the BQ123 group was lower than that in the BQ788 group.. |
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