Effects Of Genipin Scleral Crosslinking On Experimental Form-deprivation Myopia In Rabbits

At present,the population of myopia is increasing,myopia has become a common social phenomenon,most of the development of myopia is mainly in school age,but basically stable in adulthood.High myopia is also called pathological myopia,which results in prolonged axis,posterior scleral grape swelling and increased blindness rate of myopia.As a result,tens of millions of Chinese people have significantly reduced their quality of life and consumed a lot of social resources.Therefore,finding a safe and effective treatment for myopia has increasingly become an important issue in the field of life medicine science that needs to be solved.Objective: To investigate the effect of subcapsular injection of Tenon's after geniapine on the formation and development of experimental formdeprivation myopia in rabbits.Methods: Forty young New Zealand rabbits of 14 days old,male and female,were used to measure the diopter of both eyes.The right eye was used as the experimental eye and the left eye as the control eye.At room temperature 20-23 ?,natural light is used during the day,and the cycle ratio of illumination to darkness is 12h: 12 h.Group A(n = 20)were randomly divided into group A(n = 20)and group A(n = 20).The experimental eyes were treated with simple suture of eyelid suture group B as experimental group.The experimental eyes were treated with Tenon's subcapsular injection of 0.5mmol/L genipine after eyelid suture,and the suture of eyelid was opened on the 60 th day.Diopter,length of vitreous cavity,length of eye axis,flash electroretinogram were measured.The sclera at 1:00 and 7:00 on the nose of the experimental eye and the corresponding sclera of the control eye should be made into scleral strips of 5mm*10mm.The thickness and elastic modulus,creep rate,limit stress and limit strain of the sclera were measured.The changes of retina,optic nerve and sclera were observed with optical microscope.The paired t test and independent sample t test were analyzed by SPSS20.0 software.Results:1.refraction and axial lengthThere was no significant difference in diopter and axial length between the experimental eyes and the control eyes before the shape deprivation test.Before and after the experiment,there were significant differences in axial length and vitreous cavity length between right and left eyes in group A and B.After 60 days of form deprivation,the axial length of the control group(left eye)increased and the degree of degree increased to the degree of positive vision.The axial length of experimental eyes(right eye)in group A increased significantly,and the diopter increased to the degree of myopia.In group B,the number of refraction,length of vitreous cavity and axial length of eyes tended to face up to the degree of emmetropia and the length of eye axis increased compared with the control eyes.In addition,the length of vitreous cavity increased,the difference was not statistically significant.Compared with group B,the diopter number,length of vitreous cavity and axial length of experimental eyes in group A were significantly different from those in group B.2.The results of flash electroretinogram:After 60 days of experiment,the results showed that there was no difference between experimental eyes and contralateral eyes in group A and group B,and there was a comparison between experimental eyes of group A and experimental eyes of group B.There were no significant changes in amplitude and latency of dark adaptation,dark adaptation 3.0 and light adaptation 3.0 a.3.Biomechanical examination:The scleral thickness of experimental eyes and control eyes in group A and B was not statistically significant(P > 0.05).In the biomechanical experiment,the limit stress,the limit strain,the creep rate and the elastic modulus of group A were reduced by 38.97%,11.43%,16.18%,22.17%,39.27%and 39.27%,respectively.The creep rate decreased 17.88%,and the elastic modulus increased 35.70%.All the experimental eyes and the control eyes were compared with each other by paired t test(P<0.05).4.Histopathological examination:There was no change in cornea,conjunctiva and sclera in group B,and no significant change was found in the injection site of conjunctiva,fascia and sclera in experimental eyes of group B compared with the control eyes.The eye specimens of group A were stained with HE under light microscope,and the retina was found.The structure of sclera,muscle fiber and optic nerve was clear,no necrosis or degeneration was found,there was no obvious abnormality in the layer of optic cell,the collagenous fibers and muscle fibers of sclera were arranged more neatly,and the nerve fibers were arranged neatly.No inflammatory cell infiltration was observed in all tissues.Conclusion: subcapsular injection of geniapine after Tenon's is safe and effective for collagen crosslinking in the sclera of young rabbits,and can significantly increase the biomechanical strength of sclera and prevent the development of myopia in animal model.It provides a new idea and feasibility for the prevention and treatment of myopia in the future.