Study On Measurement And Clinical Significance Of Serum Myelin Basic Protein And Acyl-CoA:Lysocardiolipin Acyltransferase 1 In Patients With Diabetic Peripheral Neuropathy

Objective: To analyze the relationship between myelin basic protein(MBP),Acyl-Co A: lysocardiolipin acyltransferase 1(ALCAT1)and diabetic peripheral neuropathy by measuring the serum levels of MBP and ALCAT1 in normal group,simple diabetic group and diabetic patients with peripheral neuropathy group,so as to further explore the diagnositic significance of the serum MBP and ALCAT1 in early diabetic patients with peripheral neuropathy.Methods: 1.A total of 109 patients with type 2 diabetes who were hospitalized in the Department of Endocrinology of Lanzhou General Hospital of PLA from March 1,2017 to October 31,2017 and met the inclusion criteria were selected,according to whether there is any neuropathy or not,and divided into simple diabetic groups of 59 cases(NDPN group,31 males and 28 females),50 patients with peripheral neuropathy(DPN group,24 males and 26 females),40 healthy volunteers at the same time at our physical examination center(NC group,male 20 cases,female 20 cases)for the normal control group as the object of study.The patients with diabetic peripheral neuropathy were divided into three subgroups(mild DPN group,moderate DPN Group,severe DPN group)according to the TCSS,and compared the levels of serum MBP and ALCAT1 in each subgroup.2.Record the basic information of the selected objects,measure height and weight,calculate BMI,collect venous blood,measure fasting blood glucose(FPG).The glycosylated hemoglobin(HbA1c),superoxide dismutase(SOD),high-density lipoprotein(HDL-C),low-density lipoprotein(LDL-C),total cholesterol(TC),triglyceride(TG),aspartate aminotransferase(AST),alanine aminotransferase(ALT),urea(UREA),serum uric acid(UA),creatinine(CRE)were measured.3.The contents of ALCAT1 and MBP in sera were measured by using enzyme-linked immunosorbent assay(ELISA),and the levels of MBP and ALCAT1 in the patients with diabetic peripheral neuropathy group,simple diabetes group and normal control group were compared each other.4.Using SPSS20.0 statistic software to analyze the datas,the difference of P <0.05 is statistically significant.Results:1.Comparison of three groups of serum MBP levels:The serum MBP in DPN group was significantly higher than that in NDPN group(P<0.05)and NC group(P<0.01).There was no significant difference in serum MBP level between NDPN group and NC group(P>0.05).2.The Pearson correlation analysis of serum MBP with other clinical indicators and biochemical indicators showed:MBP levels positively correlated with diabetes duration and FPG(r=0.227,P=0.005;r=0.266,P=0.043).However,there were no significant correlation with HbA1 c,GSP,TC,TG,LDL-C,HDL-C,age,height,weight,BMI,ALT,AST,UA,UREA,CRE,SOD;3.Comparison of serum MBP in DPN patients with different lesion levels: The level of MBP in severe DPN group was higher than that in moderate DPN group(P<0.05).The level of MBP in moderate DPN group was higher than that in mild DPN group(P<0.05).The level of MBP in severe DPN group was higher than that in mild DPN group(P<0.01).4.Comparison of three groups of serum ALCAT1 levels:Serum ALCAT1 in DPN group was significantly higher than that in NDPN group(P<0.05)and NC group(P<0.01).There was no significant difference in serum ALCAT1 between NDPN group and NC group(P>0.05).5.The Pearson correlation analysis of serum ALCAT1 with other clinical indicators and biochemical indicators showed: Serum ALCAT1 levels were negatively correlated with diabetes duration and FPG(r=0.027,P=0.001;r=0.358,P=0.045),negative correlation with SOD(r=-0.294,P=0.022),However,there were no significant correlation withHbA1 c,GSP,TC,TG,LDL-C,HDL-C,age,height,weight,duration,BMI,ALT,AST,UREA,CRE,UA;6.Comparison of serum ALCAT1 levels in DPN patients with different lesions: ALCAT1 levels in severe DPN group were higher than those in moderate DPN group(P<0.05).ALCAT1 levels in the moderate DPN group were higher than those in the mild DPN group(P<0.05).ALCAT1 levels in severe DPN group were higher than thos-e in mild DPN group(P<0.01).Conclusion:1.The serum MBP and ALCAT1 have a diagnosed value for the diabetic peripheral neuropathy.2.With the increase of diabetic peripheral neuropathy,the contents of the serum MBP and ALCAT1 increased significantly,suggesting that the levels of the serum MBP and ALCAT1 can be used as good indicators to determine the severity of diabetic peripheral neuropathy.3.Serum MBP and ALCAT1 are expected to be a biochemical indicator for the early diagnosis or judgment of the severity of diabetic peripheral neuropathy.