Changes Fibroblast Growth Factor-21 Expression Of Levels In Mice Kidneys With Obesity Related Glomerulopathy

Objective:By building the model of obesity-related Glomerulopathy mice,detected the expression level of fibroblast growth factor-21 in mice kidney between control group and Obesity Related Glomerulopathy group,to discuss what Fibroblast Growth Factor-21 play role in the pathogenesis of ORG.Methods:Based on their body weight,16 SD mice that are SPF healthy males were randomly divided into 2 groups: the normal control group(8 mice)fed with common food and the ORG model group(8 mice)fed with fat-enriched diets.The mice were sacrificed at the end of the 12 th week,and their kidneys were taken out,and Gather specimens,ficing,dehydrated,paraffin imbedding,sectioning and staining.According to the principle of antigen and antibody specificity combined and histochemistry of color reaction,Immunohistochemistry was used.This study adopts the Streptomyces avidin peroxidase-link method to qualitatively,positioning and semi-quantitatively detect fibroblast growth factor-21 protein expression;To use the Image Pro Plus 6.0 Image analysis system for semi-quantitative analysis;The findings were analyzed by using SPSS21.0 software.Results:After 12 weekend,Compared with the control group[(29.39±1.25)],ORG model group mice body quality[(34.08±1.06)g] is increased,the difference was statistically significant(t =-8.09,P<0.05);HE staining showed that,compared with Control group,the ORG group kidney appear glomerular hypertrophy and the mesangial cell hyperplasia;Compared with the contol group,ORG group kidney fibroblast growth factor-21 tan positive expression of grains is incresed.Compared with the control group,qualitative FGF21 expression in ORG model group were significantly increased detected by immunohistochemistry mainly in glomerular cell cytoplasm,a small number of expression in renal tubular epithelial cells.The average absorbance value of the the model ORG group and control group was 0.228±0.667,0.010±0.011,respectively.The difference was statistically significant(P<0.05).Conclusions:The expression of growth factor-21 in kidneys of ORG model mice increased which not only indicates it may be participate in Obesity Related Glomerulopathy's occurrence and development but also provide the basis to find out the mechanisimand the approach to treatment.