Aspirin Protects Mice From Acute Lung Injury Induced By Endotoxin

Objective: This experiment was designed to investigate the protective effect of aspirin on endotoxin-induced acute lung injury in mice.Methods : Forty-eight healthy C57/6J male mice(23.0-25.0 g)were randomly selected and divided into 3 groups: control group(Control,n=16);lung injury group(LPS,n = 16);Aspirin treatment group(ASP + LPS,n = 16).In this experiment,intra-air injection was used(a longitudinal incision was made in the middle of the neck of the mice,and was slowly administered with a 1 ml syringe.The neck incision was sutured and the mouse was rotated several times vertically to promote even distribution of the liquid in the lungs).LPS and ASP+LPS were injected with LPS(2.0 mg/kg,LPS was dissolved in 50 ?l PBS)by the same method as mention above,and the control group was injected with the equal volume of airway PBS.In the ASP+LPS group,ASP(200ug/g)was injected intraperitoneally 30 min before the modeling.The specimens were collected after 8 hours of modeling in each group.The blood was gained from the eyeballs,leaving the blood for several minutes,centrifuged(3500 rpm/10 min,and the supernatant was taken and stored(-80°C).IL-1??IL-10?CD62P was measured by Enzyme Linked Immuno Sorbent Assay(Elisa)after specimen collection completed;animals were then intubated(a V-shaped incision was made at the administration site,an indwelling needle was inserted,a thin wire was ligated distally,and care was taken into a single lung),and 1.5 ml of PBS was lavaged 3 times repeatedly,and the alveolar lavage fluid was collected with a 2.5ml EP tube,centrifuged(-4°C,3500 rpm/10 min),and the supernatant was taken and stored for examination(-80°C).The automatic chemiluminescence method was used to measure the lavage fluid after Protein concentration;lung tissue of mice was taken,HE staining was used to observe the morphological changes of the lungs,and lung injury scores were performed.Results : In the control group,alveolar structure was intact in the lungs,and the alveolar septum was finely meshed.The alveolar stroma was not congested and swollen.LPS-induced alveolar wall thickening and widening were observed in the lungs of mice,and a large number of lung tissues were observed.Alveolar collapse,pulmonary interstitial hyperemia,edema,alveolar and pulmonary interstitial infiltration of a large number of inflammatory cells,alveolar telangiectasia,hyperemia;LPS mice,ASP+LPS mice lung tissue inflammatory cells infiltration,alveolar wall Capillary congestion reduces.Lung injury scores were significantly higher in the LPS group than in the Control group(p<0.05),and lung tissue scores in the ASP+LPS group were significantly lower(p<0.05).The difference was statistically significant.LPS group and Control group,LPS group BALF protein content increased,inflammation index IL-1?,IL-1 0 also increased significantly(P<0.05),platelet activation marker CD62 P expression increased;LPS group and ASP +LPS group compared with,the protein content of BALF in LPS group was increased,IL-1? and IL-10 also repeatedly significantly increased(P<0.05),and the platelet activation marker CD62 P increased obviously.Conclusions: Aspirin can reduce the degree of lung injury in LPS-induced acute lung injury in mice,and reduce the serum levels of IL-1?,IL-10,and serum platelet activation marker CD62 P in LPS mice.Therefore,ASP has a protective effect against endotoxin-induced acute lung injury in mice.