Differential Analysis Of Immune Memory In Mice Infected With Different Plasmodium

Objective: Malaria caused by plasmodium infection is one of the most serious infectious diseases of protozoa which is harmful to human health,Although great efforts have been made to control malaria in the malaria endemic area,But the emergence of resistant malaria parasites and insecticide resistant mosquito vectors makes malaria control more difficult.Therefore,the development of an effective malaria vaccine has become an important issue to be solved.Different from some other infectious diseases,malaria most notable feature is the repeated infection exsit,The cause of the phenomenon are not only related with variation of protozoa,The more important reason is that the body cannot establish a lasting and effective immune memory.Immune memory is the basis of the design and application of malaria vaccine?Vaccine against malaria,although T cells mediated immunity plays a very important role,but at present application of vaccines,most of which is to induce memory B cells produce antibodies,lasting,in order to protect the body from infectin of pathogenic microorganism,Long-lived plasma cells and memory B cells are the major cells that long-term immunity and immune memory in mammals,While the antibody as an important part of acquired immunity,specific for malaria preerythrocytic stage antigen,to prevent infection of merozoite new red blood cells,and enhance the phagocytic function of mononuclear macrophage.This experiment by creating Py.17 XNL and Pb.ANKA infected C57BL/6 mice model,The characteristics of immune memory in the process of reinfection were observed.And observe the malaria infection status,response patterns of activation in T cells and its subsets in the process,the differences between different malaria immune memory process,the difference between the immune response pattern and malaria infection,in order to provide new ideas and target for the development of an effective malaria vaccine.Methods: Create Non lethal Plasmodium yoelii 17 XNL,Plasmodium berghei ANKA infected C57BL/6 mice model by experimental method,flow cytometry,Elisa,Real-Time PCR,to observe the early infection of two rodent malaria model immunity cell and its subsets activation and immune response pattern,and the value of re-infection antibody secretion and Appreciation of the situation of memory B cells and long-lived plasma cells.The role of clear memory B cells and long-lived plasma cells in the establishment and maintenance of immune memory in malaria.Results: 1.When Py.171 XNL and Pb.ANKA groups were homologous again,Py.17 XNL group had transient hyponatremia in the blood,and the survival time was longer than that in Pb.ANKA group,while Pb.ANKA group all felt and died again 2,Py.171 XNL group and Pb.ANKA group were homologous again after total IgG levels were elevated in Py.17 XNL group than in the mouse blood and feeling before the mouse spleen cells secrete IgG memory B cells and the secretion of IgG plasma cell percentage increased significantly again feeling before,the difference was statistically significant,while the Pb.ANKA group was higher than that before feeling before has been increased,but the differences was not statistically significant,and after the total IgG level in blood,plasma cell percentage of IgG secreted by memory B cells and IgG secretion of mouse spleen cells in Py.17 XNL group than in Pb.ANKA group compared with the increase,the difference was statistically significant 3.Two groups of different rat malaria infection process,Pb.ANKA group mainly manifested as IL-1,TNF-? and other inflammatory factors increased inflammatory reaction,while Py.17 XNL group mainly manifested as TH2 and TfH type protective immune response,stimulating the body to produce protective antibodies.Conclusion: 1?Compared with the homologous reinfection of Pb.ANKA,C57BL/6 mice could resist the homologous reinfection of Py.17 XNL.2?In the process of C57BL/6 mice resistance to Py.17 XNL re-infection,memory T cells,memory B cells,secreted IgG plasma cells and specific antibodies IgG,IgG1 and IgG2 a are mainly involved in this response process.3?Th1,Th2 and TfH cell subsets mediate the resistance of C57BL/6 mice to re infection of Py.17 XNL.