Study On Chemical Constituents And Potential Anti-depressive Activity Of The Seeds Of Caesalpinia Sappan L.

Caesalpinia sappan L.is a species in Caesalpinia?Leguminosae?.The dried heartwood of C.sappan is a traditional medicine,which has been used for years in China.It can promote blood circulation,antibacterial and anti-inflammatory function.This thesis focuses on the chemical investigation and potential anti-depressive activities of cassane diterpenoids isolated from the seeds of C.sappan.The compounds were isolated and purified by repeatedly chromatography with silica gel,Sephadex LH-20,ODS?C-8 and C-18?,and MCI.The structures were identified by IR,NMR and MS methods.23 compounds were isolated among which18 compounds were identified.They are 9,10-O-isopmpyllidene-?12 Z?-en-octadecaoic acetate?CS-01?,?-sitosterol?CS-02?,24-ethyl-5,22-cholestadien-3-beta-ol?CS-03?,phanginin A?CS-04?,phanginin L?CS-05?,phanginin C?CS-06?,18-acetoxycassa-12,15-diene-3-one?CS-07?,apigenin?CS-08?,vanillic acid?CS-09?,biochanin A?CS-10?,daucosterol?CS-11?,?-amyrin?CS-12?,spathulenol?CS-13?,cycloeucalenol?CS-14?,?-sitosterol palmitate?CS-15?,cycloart-23-ene-3?,25-diol?CS-16?,quercetin?CS-17?,phanginin H?CS-18?,caesalppans G?CS-19?.Among them,CS-01 is a new coumpound,CS-06,CS-08,CS-12,CS-13 and CS-14 were isolated from this plant for the first time.Neuroprotective effect of cassane diterpoids was studied in corticosterone-induced PC-12 cell neurotoxicity.Cell viability was assessed by MTT assay.The released amount of lactate dehydrogenase?LDH?and[Ca²⁺]_i concentration were measured.The results indicated that the compounds extracted from the seeds of C.sappan could bring neuroprotective effect,proving a possible action pathway by decreasing LDH release and[Ca²⁺]_i concentration.