Investigation On The Active Substance Of Scilla Scilloides With The Capture Of Dicarbonyl Compounds

Diabetes is a clinical syndrome characterized by chronic hyperglycemia caused by absolute or relative deficiency of insulin.Diabetes and its chronic complications have become the third leading cause of death in humans.Related studies showed that advanced glycation end products(AGEs)play an important role in the complications of diabetes.They can directly obtain proteins and cause cross-linking of proteins to affect their structure and function,stimulate key cell signaling pathways and release cytokines,inflammatory factors,etc.,leading to the occurrence of diabetic complications.According to reports in the literature,dicarbonyl compounds are key intermediates for the formation of AGEs,which can reduce the generation of diabetic complications by effectively reducing the amount of dicarbonyl compounds.Studies have shown that compounds homoisoflavonoids have the function of capturing dicarbonyl compounds and the Scilla scilloides have been reported to have a large amount of homoisoflavonoids.But it has not been reported whether the extract of Scilla scilloides has the activity of capturing dicarbonyl.We selected Scilla scilloides as a research object to study the material basis and mechanism of its capture of dicarbonyl compounds.Firstly,the active screening was performed on the eluted fractions of the crude extract and the macroporous resin using the classical protein non-enzymatic glycosylation activity model.The results showed that the crude extract of Scilla sinensis was obtained at 80 ?g/m L.And the elution sites all showed good protein non-enzymatic glycosylation inhibitory activity,the inhibition rates were 52.38%,70.10%,92.76%,82.10%,66.10%,76.57%.When the positive drug aminoguanidine concentration was 500 m M,the inhibition rate was 41.90 %.Then,an activity model for capture of dicarbonyl compounds was established.Using a model to screen the elution sites of different concentrations of ethanol in crude extracts.The results showed that crude extract 95% ethanol elution site has good activity for capturing dicarbonyl compounds.The Scilla scilloides are separated on a silica gel column chromatography and RP-18-reversed-phase silica gel column.After separation processes by preparative HPLC of active fractions,eleven compounds were obtained,and their structure were identified as scillanostaside M-1(1),scillanostaside M-2(2),scillanostaside M-3(3),scillanostaside M-4(4),5-hydroxy-3-(4-hydroxybenzyl)-7,8-dimethoxychroman-4-one(5),5-(hydroxymethyl)furan-2-carbaldehyde(6),scillascilloside E-2(7),scillasclloside E-3(8),scillanostaside A(9),scillanoside L-1(10),scillascilloside G-1(11).Invistigate the compounds isolated from Scilla scilloides extraction such as 3-(4-Hydroxybenzyl)-5-Hydroxy-7,8-Dimethoxychroman-4-one(5)capture of dicarbonyl compounds activity in vitro.Results showed that when the concentration of the compound is 0.32 m M,the capture activity of the dicarbonyl compound is 44.48%.This subject researched the chemical composition of Scilla scilloides,provide a scientific basis for the comprehensive development and utilization of Scilla scilloides resources.