The Effects Of CTRP9 On Cardiocyte Hypertrophy Induced By High Glucose In Vitro

Objective: This study aimed to investigate the effect of C1q/tumor necrosis factor-related protein 9(CTRP9)on high glucose-induced cardiomyocyte hypertrophy.Methods:1.Rat cardiomyocyte were cultured in this study,?-actin was used to identify primary cardiomyocyte.2.A model of high glucose-induced cardiomyocyte hypertrophy was made in vitro.Rat cardiomyocyte were randomly divided into 5 groups: the control group,CTRP9 group,high glucose group,high glucose with CTRP9(treatment at the same time)group,high glucose with CTRP9(pretreatment)group.3.The surface area of cardiomyocyte was determined by professional Image analysis software.The protein expression of atrial natriuretic peptide(ANP)and?-myosin heavy chain(?-MHC)in each group were quantified by western blot method.4.The gene level of Beclin 1 and Atg 12 in each group were determined by real-time PCR.the western blot method was used to detect the protein level of Beclin 1 and Atg 12.Results:1.The model of high glucose-induced cardiomyocyte hypertrophy was made:Compared with that in the control group,the cell surface area and the protein level of ANP and ?-MHC in the three different concentrations(15,25,40mM)of high glucose group increased significantly,peaked at 25 mM,the cell surface area and the protein level of ANP and beta MHC were less than 25 mM group than that in 15 mM and 40 mM group,there are significant statistical difference(P < 0.05).2.The inhibitory effect of CTRP9 on high glucose-induced cardiomyocyte hypertrophy: compared to the control group,there was no change in the cell surface area and the protein level of ANP and?-MHC(P>0.05).Compared to the high glucose group,the cell surface area and the protein level of ANP and?-MHC decreased significantly than that in high glucose with CTRP9(treatment at the same time)group and in high glucose with CTRP9(pretreatment)group,there was significant statistical difference(P < 0.05).3.The result of western blot showed that: compared to the control group,there was no change in the gene and protein level of autophagy related protein such as Beclin 1 and Atg 12 in CTRP9 group(P>0.05).high glucose surpressd the expression of autophagy related protein.compared to the high glucose group,the gene and protein level of autophagy related protein such as Beclin 1and Atg 12 increased significantly than that in the high glucose with CTRP9(treatment at the same time)group and in high glucose with CTRP9(pretreatment)group,there was significant statistical difference(P < 0.05).4.The result of real-time PCR showed that: compared to the control group,there was no change in the gene and protein level of autophagy related protein such as Beclin 1 and Atg 12 in CTRP9 group(P>0.05).high glucose surpressd the expression of autophagy related protein.compared to the high glucose group,the gene and protein level of autophagy related protein such as Beclin 1 and Atg 12 increased significantly than that in the high glucose with CTRP9(treatment at the same time)group and in high glucose with CTRP9(pretreatment)group,there was significant statistical difference(P < 0.05).Conclusion:CTRP9 can inhibit high glucose-induced cardiomyocyte hypertrophy,and this effect may be mediated by autophagy.