Effect Of Glucagon-like Peptide 1 Receptor Agonist In Sesn2/AMPK/mTOR Signaling Pathway In Liver Of Obese Rats Induced By High-fat Diet

Objective: To explored the intervention of glucagon-like peptide 1 agonist liraglutide in Sesn2/AMPK/m TOR signaling pathway in liver of high-fat diet rats.Methods: Male SD rats were divided into normal chow group(NC=12)and high-fat diet group(HF=33).The rats in HF group were fed with high-fat diet for 12 weeks to induce obese,then the model rats were divided into 4 subgroups(HF,LG,MG,HG)treated with various doses of liraglutide(0,50,100,and 200 ?g/kg)hypodermic injection twice a day for 4 weeks.The body weight and epididymal-fat index of the rats at 16 th week were measured.The liver tissue fatty degeneration was observed.The protein levels of Sesn2,AMPK,p-AMPK,m TOR,p-m TOR were determined by Western blot.Results: 1.The weight of rats in HF group was obviously higher than that in NC group(P<0.01).Compared with NC group,the protein level of Sesn2 was significantly decreased in HF group(P<0.01),The level of p-AMPK/AMPK was significantly decreased(P<0.01),The level of p-m TOR/m TOR was no changed(P>0.05).2.After treatment with liraglutide for 4-week,the body weight of the rats in LG,MG and HG groups was obviously lower than that in HF group(P<0.01),and epididymal-fat index of the rats in MG and HG groups was obviously lower than that in HF group(P<0.01).The protein level of Sesn2 in HG group was obviously higher than that in HF group(P<0.01).The level of p-AMPK/AMPK was increased significantly in MG and HG groups(P<0.01).The level of p-m TOR/m TOR was significantly decreased in LG,MG and HG groups(P<0.01).Conclusion:Glucagon-like peptide 1 agonist liraglutide affects energy metabolism and improves the state of obesity through Sesn2/AMPK/m TOR signaling pathway.