The Clinical Significance And Levels Of IL-35 In Serum Of Patients Withprimary Sjogren's Syndrome

Objective: To evaluate the level of interleukin-35(IL-35)in the serum of patients with primary Sjogren's syndrome(pSS)and its relationship between clinical parameters such as disease activity,autoantibodies and system fatigue and hence to provide a scientific basis for the treatment of pSS.Methods and materials:A total of 176 patients with pSS and 60 healthy controls from the Department of Rheumatology and Immunology,First Affiliated Hospital of China Medical University,were recruited from January 2016 to April 2017 as the experimental group and healthy control group.The laboratory parameters include anti-SSA antibody,anti-SSB antibody,anti-Ro-52 antibody,RF,ESR,Ig-G,TgAb,TPOAb,ANA,AMA,SMA and clinical features include: lung damage,kidney damage,thyroid and liver involvement.Serum IL-35 levels in patients with pSS and healthy controls were measured by enzyme linked immunosorbent assay(ELISA).The disease activity of pSS were scored by disease index system(ESSDAI)recommended by the European Association for the Prevention of Rheumatology(EULAR)in 2009.Results:1.Serum levels of IL-35 in patients with pSS were significantly higher than those in healthy controls,the difference was statistically significant(P=0.021).2.Serum levels of IL-35 in patients with pSS were positively correlated with ESR,ESSDAI and IgG,with statistical significance differences(r =0.158,P=0.035;r =0.213,P=0.004;r=0.426,P<0.0001respectively).3.Serum levels of IL-35 in patients with pSS are associated with RF.In RF positive group,serum IL-35 levels was significantly higher than those in RF negative group,the difference was statistically significant(P = 0.045).But no correlation with anti-SSA antibody,anti-SSB antibody,anti-Ro-52 antibody(P>0.05);4.In pSS patients,the serum IL-35 levels in the pSS with ILD were higher than those without ILD,the difference was statistically significant(P = 0.0002).However,there were no correlation between serum IL-35 levels and complications of pSS including Renault,salivary gland swelling,autoimmune thyroid disease,autoimmune hepatitis and renal tubular acidosis(P>0.05).Conclusion: 1.Serum levels of IL-35 in patients with pSS were significantly higher thanthose in healthy subjects and were positively correlated with disease activity.2.IL-35 may participate in the occurrence and development of pSS,and play a role in the occurrence and development of ILD.