TGF-?1 Induced Fascin1 Expression Facilitates The Migration And Invasion Of Kidney Carcinoma Cells Through ERK And JNK Signaling Pathways

Objective:To investigate whether Fascin1 plays a crucial role in TGF-?1-facilitated invasion and migration of renal cancer cells.Methods:Real-time PCR and Western blotting were used to test the Fascin1 expression after TGF-?1 treatment(10 ng/ml)in 769-p and OSRC cells.Cytoskeleton staining was used to test the change of cytoskeleton.Cell migration and invasion changes were measured by Wound-healing and Transwell methods.Fascin1 was silenced using the small interfering RNA(si RNA)technique.Results:The results indicate that m RNA and protein levels of Fascin1 were dramatically increased after treatment with 10 ng/ml TGF-?1 in 769-P and OSRC cells(P<0.05).The TGF-?1 also promotes the occurrence of EMT(Epithelial-Mesenchymal Transition)and the invasive and migratory capabilities of the two cell lines after treatment with 10 ng/ml TGF-?1(P<0.05).In addition,Fascin1 si RNA dramatically attenuated the invasiveness and migration induced by TGF-?1.Furthermore,we identified that specific inhibitors of ERK and JNK signaling pathways,FR180204 and SP600125,can suppress TGF-?1-induced Fascin1 expression.Conclusion:In conclusion,these results reveal that Fascin1 is an important mediator of TGF-?1-induced invasion and migration of kidney cancer cells through ERK and JNK signal pathways.