Effects Of Different Peripheral Nerve Functional Reconstruction Models On Skeletal Muscle

Objective:To study the effects of different peripheral nerve-muscle functional reconstruction procedures and postoperative rehabilitation training on neuromuscular remodeling in rats.Methods: The SD rats were randomly divided into two groups,one each separated from the ulnar nerve,one group implanted the proximal part of the ulnar nerve into the biceps and cut off the musculocutaneous nerve that dominates the biceps muscle to construct the targeted neuromuscular function Reconstructed(Targeted Muscle Reinnervation,TMR)rat model.The other group implanted biceps femoris proximal to the ruptured ulnar nerve,but kept the original musculocutaneous nerve that dominated biceps muscle to construct a rat model of Targeted Nerve Reinnervation(TNR).After modeling,two groups of rats were given free or treadmill training respectively.At 4 weeks and 12 weeks after the model establishment,the biceps brachii muscle tension was measured and the maintenance rate of biceps muscle wet weight was calculated.The intramuscular nerve and motor endplate staining were used to observe the biceps brachii Intramuscular nerve growth,evaluation of two kinds of operation and treadmill training on peripheral nerve-muscle function reconstruction.Results:1.Effects of two kinds of operation on peripheral nerve-muscular function reconstruction: 1)After intramedullary biceps intramedullary nerve staining,the muscular tissue of biceps was transparent or translucent,intramuscular The nerves were blue,the distribution and the direction were clear,but there was no musculocutaneous nerve in the TMR group,and the musculocutaneous nerve was seen in each group of TNR.Prompt two surgical modeling success;2)In the free-moving rat model,the maximum uniaxual contractility of the biceps in each group was significantly higher than that of the TMR group(P <0.05).The maintenance of biceps muscle wet weight in each group of TNR wasgreater than TMR groups(P <0.05).The number of biceps brachii motor endplate in each group was more than that in TMR group(P <0.05),which suggested that TNR was more beneficial to peripheral nerve-muscle function reconstruction than TMR.2.The effect of treadmill training on postoperative peripheral nerve-muscle function reconstruction: In TMR group and TNR group,the maximum systolic force of biceps in each group was significantly higher than that of free running(P <0.05).The maintenance rate of biceps muscle wet weight in treadmill training group was higher than that of free treadmill exercise group(P <0.05).The number of biceps motor endplate in treadmill training group was more than that of free treadmill exercise group Activities of each group more(P<0.05);It is prompted that the treadmill training is conducive to postoperative peripheral nerve-muscle function recovery.Conclusion:1.TMR and TNR surgery can achieve peripheral nerve-muscle function reconstruction,but TNR surgery is more conducive to peripheral nerve-muscle function reconstruction than TMR;2.Treadmill training is beneficial to postoperative peripheral nerve-Muscle function recovery.