| It is often difficult for soft tissues to self-repair severe injuries,and it is impossible to achieve reconstruction of the organizational structure and functional recovery.Allograft is an important program that helps organizations to repair and rebuild clinically.A variety of biosynthetic materials are currently used as tissue engineering scaffolds for tissue repair.However,these synthetic materials generally have poor cell affinity,and cells on the surface of the materials have weak adhesion,growth and differentiation.On the contrary,natural collagen materials have become an ideal tissue engineering scaffold material because of its unique biological activity factor.Due to its rich collagen content and good mechanical properties,tendon tissues have become a hot topic in the research of tissue engineering scaffolds.Current studies have found that the combination of decellularized tendon scaffolds with BMSCs transplantation can promote tendon injury repair,and the arrangement of collagen fibers plays an important role in stem cell differentiation.Therefore,in this study,we prepared aligned and random acellular scaffolds by rat tail tendon tissues.In order to investigate the regulation effect of collagen fiber arrangement on the differentiation of BMSCs,the scaffolds seeded with BMSCs were incubated with different serum concentrations.We hope tendon ECM can regulate the biological behavior to directed differentiation of BMSCs,promote regeneration of tendons.In this study,we found that: 1)The proliferation activity of BMSCs seeded on aligned/random collagen scaffolds were good;2)BMSCs were elongated and aligned along the collagen fibers on the aligned collagen fiber scaffold;3)Aligned/random collagen scaffolds can significantly promote the expression of TNMD of BMSCs,and the promotion of 2% FBS culture is stronger than that of 10% FBS culture;4)The promotion of aligned collagen scaffolds is stronger than that of random collagen scaffolds.The above results indicate that directed collagen scaffolds can induce differentiation of BMSCs into tenocytes at low serum concentrations.The results of this study are expected to provide a new theoretical basis for the selection of tendon tissue engineering scaffolds. |
