Protective Effects Of Oleuropein On Oxidative Stress In Parkinson Model Mice And Neurobehavioral Effects

In neurodegenerative diseases,Parkinson's disease is the second largest disease after Alzheimer's disease,with a prevalence of 2% among people over the age of 65.In traditional understanding,Parkinson's disease is considered to be an idiopathic or sporadic disease.It is a disease characterized by loss of dopaminergic neurons.Static tremor,myotonic,slow motion and unstable posture are the main clinical manifestations.There is no good cure for Parkinson's disease.Oxidative stress and neuroinflammation have played an important role in the pathological process of Parkinson's disease,and the improvement of oxidative stress damage has become an important means for the treatment of Parkinson's disease.Oleuropein(OE)is a bioactive compound.It is a benzol structure linked to benzene ring from the basic ethanol structure of the isolated grass.It has the function of antioxidant stress.The latest study in the Mediterranean showed that the observation of local people's diet was the protective effect of olidoside on the heart and the brain;another study found that olidoside could reduce the risk of new diabetes,and two studies showed that Oleuropein produced a therapeutic effect through antioxidant stress.We conclude that Oleuropein may be an effective drug for the treatment of Parkinson's disease.Objective:To study the protective effect of Oleuropein on oxidative stress injury in Parkinson model mice and its neurobehavioral effects.Method:A total of 30 C57 male C57 male mice were randomly divided into 5 groups,with 6rats in each group: blank control group,model group,positive drug group(Mei dosa)group,olive bitter glucoside low dose group and Oleuropein high dose group.General observation of experimental animals: All mice were weighed;animal neurobehavioral tests: rotating rod test and open field test.After the mice were tested for the neurobehavioral test,the Nrf2 expression level in the brain of each group was detected by intraperitoneal injection of chloral hydrate,and the damage of dopamine neurons in the substantia nigra was observed by immunohistochemical staining of the substantia nigra part.Result:1.OE did not improve the body weight of Parkinson mice.There was no significant difference between the model group(24.74 + 0.85g)and the low dose group(25.02 +0.61g)of Oleuropein(p > 0.05),and there was no significant difference between the model group(24.74 + 0.85g)and the high dose group(24.96 + 0.52g)(p> 0.05).2.Oleuropein(OE)could improve its exercise ability.In the rotation bar experiment,the low dose of Oleuropein increased to 51.02 + 7.38 s in the stick time,and we found that the high dose of Oleuropein was also prolonged at the bar time(p< 0.05).We observed in the open field test.The exercise path of Parkinson mice was increased to2123.48 + 21.15 mm after low dose of Oleuropein,but there was no significant difference between the high dose of Oleuropein and low dose(p > 0.05).3.the protein expression level of Nrf2 in Oleuropein low dose group and Oleuropein high dose group was higher than that in model group.4.compared with the model group,the number of dopaminergic neurons in the substantia nigra in the Oleuropein group was also significantly increased.Conclusion:In the Parkinson mouse model established by MPTP,Oleuropein(OE)enhances the expression of Nrf2 protein by activating the Keap1-Nrf2-ARE signaling pathway,which affects the initiation of a variety of downstream protective genes and alleviates oxidative stress damage.Although Oleuropein can improve the motor function of Parkinson mice,it is not clear that the therapeutic effect is related to the concentration of Oleuropein.And the drug had no significant effect on the weight of Parkinson mice.