| Objectives:SEM technique was used to characterize the surface micro-morphology of the bone in a number of individual femoral heads under different age,gender and bone density,and to analyze and summarize the surface morphology parameters of the images,in order to provide the surface morphology parameters of the human femoral head in different condition and improve the database of surface micro-morphology of the human bone,provides data support for designing biomimetic materials in the future.Methods:1.Collection of human femoral head samples:All of the femoral head samples came from the orthopaedics patients who undergoing total hip replacement in The First Affiliated Hospital of Kunming University,during 2016.11-2017.10,and exclusion and inclusion criteria were established.A total of 43 cases of postoperative femoral heads were discarded.According to age,gender and preoperative bone mineral density,the patients were divided into young and middle-aged group(40-59 years old)/old group(60-83 years old),21 cases in the male group and 22 cases in the female group;normal bone mass group(4 males and 5 females,9 cases in total);bone mass reduction group(8 males and 5 females,13 cases in total)and osteoporosis group(nine males,12 females,total 21 cases).2.Fabrication of human femoral head cancellous bone electron microscope and Characterization of the surfuce micro-morphology,The femoral head was cut from the coronal plane and placed in a CT scan,the non-necrotic area and the necrotic area were initially divided based on the radiographic image of the femoral head CT and the outline of the osteotomy surface.3.Extraction and analysis of surface morphology parameters of human femoral head,Based on the self-developed image processing method of the research group,the morphological parameters derived from the SEM images,ie,fiber orientation distribution,fiber length,and fiber diameter,were extracted.The obtained micro-morphological parameters were analyzed by independent sample t-test according to the comparison between the two groups.The comparison of three groups was performed using a complete random variance analysis,and the pairwise comparison was performed using the LSD-t pairwise comparison method for statistical analysis to obtain the reference value range and confidence interval of the morphological parameters of different genders,ages,and bone states.Results:1.Electron microscopy images of surface microscopic features grouped under different factors:1)The diameter of the mineralized collagen fiber bundles of the young and middle-aged groups is coarser than that of the elderly group,and they are closely arranged.2)The diameter of mineralized collagen fiber bundles in the male group was generally coarser than that in the female group.3)In the normal group,the mineralized collagen fiber bundles were interdigitated and the diameters were coarse,and the gap between the bone mineralized collagen fiber bundles was small;the diameter of the bone mineral collagen fiber bundles in the reduction group was smaller than that of the normal group,and the gap between the collagen fiber bundles was larger;in the osteoporosis group,the diameter of the bone mineralization collagen fiber bundle is the smallest,the arrangement is irregular,and some fiber bundles are broken or missing.2.Micro-morphological parameters grouped under different factors:1)male group:mineralized collagen fiber bundle diameter 1.91 ±0.65um,length 21.11±7.95um,angle 55.78±15.40°;female group:mineralized collagen fiber bundle diameter 1.76±0.92um,Length 22.57±10.20um,angle 60.67±12.27°.2)Young and middle-aged group:mineralized collagen fiber bundle diameter 2.22±0.25um,length 27.02±12.25um,angle 46.85±7.53°;elderly group:mineralized collagen fiber bundle diameter 1.76±0.62um,length 20.89±8.11um,angle 60.51±13.99°;3)Osteoporosis group:mineralized collagen fiber bundle diameter 1.57±0.46um,length 19.14±7.98um,angle 66.67±9.40°;bone mass reduction group:mineralization Collagen fiber bundle diameter 2.02±0.71um,length 23.10±7.89um,angle 51.66±14.78°;normal bone mass group:mineralized collagen fiber bundle diameter 2.22±0.25um,length 27.02±12.25um,angle 46.85±7.53°.3.After statistical analysis,the difference in diameter between the osteoporosis group,1)the bone loss reduction group,and the bone loss reduction group was statistically significant(P<0.001).After further comparison,the osteoporosis group and the bone mass normal group diameter group were compared.The difference between the two groups was statistically significant(P<0.05).There was no significant difference in diameter between the other two groups(P>0.05).There was a statistically significant difference between the three groups and between the angles of the groups(P<0.05).2)There was significant difference in diameter,angle and length between the old group and the young group(P<0.05).3)There was no significant difference between the male group and the female group(P=0.019).There was no statistical difference in diameter and length.Significance(P>0.05).Conclusion:1.Scanning electron microscopy(SEM)can clearly and completely characterize the microscopic morphology of the bone surface.The use of ImageJ software's digital image module program(Directionality and Orientation J)to process electron microscopy images can accurately and reliably extract microscopic topography parameters.These two methods meet the needs of this study.2.Determined the confidence interval of the microscopic morphology(diameter,length,and angle of mineralized collagen fiber bundles)of the femoral head cancellous bone surface.3.According to the statistical analysis,there is a positive correlation between the bone mineral density and the length and length of the mineralized collagen in the microscopic topological parameters,and there is a negative correlation between the age and the angle;the morphology parameters under different conditions obtained through this study can be used in the future.The basis for judging the status of human femur skull in clinical practice. |
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