Study On Mechanism Of Delaying Renal Fibrosis By Qiguiyishen Decoction Based On MiRNA Regulation

Objective: To investigate the effect and mechanism of Qigui Yishen Decoction(QGYS)on the expression of mi R-141 in unilateral ureteral obstruction(UUO)mice models.Methods: Thirty healthy Balb/c male mice randomly divided into groups of sham-operated group(n=6),UUO group(n=6),Lotensin(25ml/kg)group(n=6),QGYS high dose(25ml/kg)group(n=6),and QGYS low dose(5ml/kg)group(n=6)were conducted UUO surgery to promote kidney fibrosis except for the 6 mice of sham operation group.After a successive 10-day medication of QGYS and Lotensin to mice by oral gavage on daily basis,all mice were killed to procure renal tissue to observe its morphology and pathology changes by HE staining.The expressions of TGF-?1,Col IV,MMP-9 were analyzed by immunohistochemical method,and the expressions of mi R141,TGF-?1 were measured by real-time PCR.Results: The obviously pathological injuries including renal interstitial fibrosis were identified by HE staining among the groups intervened with UUO,but the variance in the extent due to different administrations of QGYS and Lotensin group achieved an up-regulated expression of TGF-?1 and Col IV,and a down-regulated expression of MMP-9 by immunohistochemistry(P < 0.05),and significantly increased m RNA expression of mi R-141,and decreased m RNA expression of TGF-?1 by real-time PCR(P < 0.05).Conclusion: In UUO mouse models,QGYS gives influence to TGF-?1 and MMP-9 through inducing mi R-141 expression change to decrease abnormal accumulation of ECM,and thus inhibit the progression of renal fibrosis.