| Objective:In this experiment,we used the high-frequency welding equipment and manual silk to anastomose the colon of SD rats in vivo respectively.According to the time of colon anastomosis,the survival rate after operation,the size of colon blasting pressure and the postoperative pathological changes of colon,we tried to explore whether it is feasible to perform the high-frequency welding equipment to anastomose rat colon in vivo.Methods:(1)Model making:In this experiment,80 male 10-week-old SD rats weighing 350g were randomly divided into experimental group and control group with 40 rats in each.We used the high-frequency welding instrument to side-to-side anastomose the rat colon in experimental group,and the traditional manual silk in control group.(2)Anastomosing time:we utilized the electronic stopwatch to record two groups of anastomosing time.But the time of two groups in opening and closing abdomen was not included.(3)Survival rate:After the completion of colon anastomosis,we observed the long-term survival rate of the experimental group and the control group.(4)Burst pressure measurement:10 rats of two groups were randomly selected to be put to death after 1,2,4 weeks.And then we used a precision pressure gauge to record the value at this time.(5)Postoperative pathological changes:After the burst pressure measurement was completed,we cut the colon tissue for pathological examination 'by Masson staining.The relevant cytology and collagen fibers were observed carefully under the light microscope.(6)Statistical methods:The data from two groups of experiments were analyzed by SPSS20.0 statistical software.The means of the experimental group and the control group were compared by test,and the counting data was represented by X± S.The difference can be considered statistically significant when P<0.05.Result:(1)Anastomosing time:The average anastomosing time of experimental group using high-frequency welding instrument was(17.40±1.56)s,significantly less than the control group using manual silk(628.40 ±24.86)s.It could be considered the difference was statistically significant when P<0.01 proving that experimental group could reduce the operation process and significantly shorten the operation time.(2)Postoperative long-term survival rate:The groups of rats died within 3 days to a certain extent after operation.The experimental group had 2 deaths and the control group had 7 deaths.The postoperative long-term survival rate of the experimental group was higher than the control group,indicating that high-frequency welding had more reliable advantage.(3)Postoperative colon burst pressure:The postoperative colon burst pressure of experimental group in the first and the second week were bigger than the control group.The P<0.05 showed there was a statistically significant difference.However,two groups in the fourth week had no significant difference,indicating that the effect of anastomosis by high-frequency welding was better than the tradictional method of suture in the early and mid-term stages.(4)Postoperative pathology results:Collagen fibers have the characteristics of rigidity and toughness under the normal physiological conditions.Its quantity and density are the key factors to maintain the mechanical strength of anastomosis.Compared with the control group,the experimental group had less inflammatory response at the first week.We considered residual suture of anastomosis in the control group caused greater inflammatory response due to its non-continuity suture by manual silk.At 2 and 4 weeks,the number of collagen fibers was larger and the density was higher,suggesting that the anastomotic tension in the experimental group was greater and the resistance to mechanical pulling was stronger,which was consistent with the results of postoperative colon burst pressure in both groups.Conclusion:Combined with the anastomosis time,postoperative survival rate,the size of the colon burst pressure and postoperative pathology results,we could concluded that high-frequency welding technology can safely and effectively anastomose the colon of rats in vivo. |
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