Study On The Activation And Repair Of CD133+ Neural Stem Cells After Injury Of The Fourth Ventricle In Adult Mice

Neural stem cells(NSCs)are stem cells that with the ability of self-renewal and multiple differentiation potential.Under certain circumstances,NSCs are able to differentiate into neurons,astrocytes and oligodendrocytes,thereby participating in the neurogenesis and injury repair.Normally,adult NSCs are mostly quiescent state.Under pathological conditions,quiescent NSCs could be activated,proliferated,migrated and differentiated into new neurons and reconstruct neural circuit in damaged sites.At present,NSCs are mainly distributed in the subventricular zone of lateral ventricle and subgranular zone of the Hippocampus.We found that there has quiescent neural stem cells of CD133 in non-neurogenic region of adult mouse “fourth ventricle ventricular zone”.Therefore,the study of quiescent neural stem cells in the fourth ventricle and neurogenesis will provide greatly insight into brain function,development of the nervous system,pathogenesis of neurological diseases,and repair of central nervous system damage by endogenous neural stem cells.In this study,we used the CD133-CreERT2:R26R-Rosa26-CAG-LSL-zSGreen of traced mouse as experimental materials and explored the neurogenesis in the fourth ventricle and activation of neural stem cells and regeneration of vascular system after injury by tracing the resting neural stem cells of CD133.The results show that under normal physiological conditions,there are various types of nerve cells around the fourth ventricle,indicating that the neurogenesis in the fourth ventricle maintains normal physiological functions.Hemorrhagic brain injury in the vestibular nucleus region of the fourth ventricle in adult mice by stereotaxic.We found that not only the quiescent neural stem cells of CD133 were activated in the ependymal region of the fourth ventricle,but also the stem cells of the vascular system associated with CD133+ vascular endothelial cells and pericytes were activated.These activated stem cells can migrate to the injury site for nerve injury repair.This study is likely to provide new ideas for the treatment of brain injury,stroke and neurological diseases.