Effects Of Leptin On Endoplasmic Reticulum Stress Related Proteins After Focal Cerebral Ischemia

Object:To investigate the effect of leptin on endoplasmic reticulum stress after focal cerebral ischemia in rats.Method:40 SD rats were randomly divided into sham operation group,cerebral ischemia group and leptin group.Ischemia was induced by occluding the middle cerebral artery in rats brain using the filament occlusion method.Subcutaneous injection of leptin 3 hours before occlusion of blood vessel.Longa 5 score neurological function scale,body weight and brain edema changes were measured after 6 hours of MCAO model,and the brain was removed to detect the endoplasmic reticulum marker protein glucose-regulated protein 78(GRP78)and C/EBP-homologous protein(CHOP)by immunohistochemical method.Results:There was no difference in the body weight changes in the leptin group and ischemic group.In the leptin group,the neurological function score(1.9±0.31,P<0.05)and the degree of brain edema(3.6±0.52 mg,P<0.001)were significantly lower than those in the cerebral ischemia group.Moreover,the expression of GRP78 in leptin group was significantly higher than that in ischemia group(P<0.01),and the expression of CHOP was significantly lower than that of ischemia group(P<0.01).Conclusions:Leptin can reduce the neurological deficit and may be associated with the up regulation of GRP78 protein,down regulation of CHOP protein to weaken the endoplasmic reticulum stress caused by cerebral ischemia.