Application Of P53 In The Origin Of Serous Ovarian Cancer

OBJECTIVE :(1)Using immunohistochemical method to detect the expression of p53 protein in ovary and fallopian tube of the same serous ovarian cancer patient,Combining with clinicopathological data of patients to investigate whether there are differences in clinicopathology between p53 imprinted positive and p53 negative expression of serous ovarian carcinoma in ovary and fallopian tube.(2)To investigate the relationship between peripheral blood lymphocyte immunophenotype and clinicopathology of serous ovarian cancer,and analyze the relationship between peripheral blood lymphocyte immune typing and prognosis of serous ovarian cancer.METHODS :(1)Collected postoperative specimens of 55 patients w ith serous ovarian cancer from January 2015 to December 2017 in the Ca ncer Hospital affiliated to Guangxi Medical University(52 high-grade s erous carcinoma,3 low-grade serous carcinoma).The specimen was t aken from ovarian cancer patient's ovary and fallopian tube after ovarian tumor cell reduction operation,fallopian tube sampling include the fallopia n tube umbrella,tubal ampulla,tubal narrow section.The expression of p53 in ovarian and oviduct specimens was detected by immunohistochemi cal method,then according to the positive expression of p53 in ovary and fallopian tube,the patients with positive p53 expression in ovary and fall opian tube were divided into two groups: the patients with p53 positive e xpression in ovary and fallopian tube were divided into p53 imprinted pos itive group,ovarian and fallopian tube p53 consistent negative expression g roup as p53 imprinted negative group.HE staining morphology of ovary and fallopian tube were observed respectively.All the results were confir med by two senior pathologists of our hospital.p53 imprinted positive sp ecimens from 28 patients with serous ovarian cancer(26 cases of high gra de serous ovarian carcinoma 2 cases of low grade serous ovarian cancer),p53 imprinted negative specimens from 10 patients with serous ovarian ca ncer(9 cases of high grade serous ovarian carcinoma and 1 case of low grade serous ovarian cancer.Collected the clinical data of the two groups such as lymphocyte immunophenotype before operation?chemotherapy and recurrence after ovarian cancer reduction etc,and analysed whether there were differences in clinicopathology between the two groups.(2)Retrospective analysed 100 cases of serous ovarian cancer with peripherl blood lymphocyte immunological typing and the peripheral blood lymphocyte immunophenotyping cells before primary treatment were collected,including total T lymphocyte(CD3+)? helper lymphocyte(CD3 +CD4+)? suppressive lymphocyte(CD3+ CD8+)? the ratio of helper lymphocytes to suppressor lymphocytes(Th/Ts)? natural killer cells(CD3-CD16+CD56+)? B lymphocytes(cCD19+),then according to the pathological data,the immunophenotyping of peripheral blood lymphocytes in different groups was analysed statistically significant.RESULTS :(1)The average progression-free survival time was 8.27 ±7.90(months)in the positive group and 16.78 ±10.81(months)in the negative group,progression-free survival time in p53 imprinted positive group was shorter than that in p53 negative group(p< 0.05).(2)The tumor residues,ascites,chemotherapeutic drug reactions and lymphocyte immunophenotyping in peripheral blood were compared between p53 imprinting positive group and p53 negative group,and there were more tumor residues in p53 positive group than in p53 negative group(p<0.05).Ascites?chemotherapeutic drug reactions?peripheral blood lymphocyte immunophenotyping cells were not found to be statistically significant(p>0.05).(3)The level of Th/Ts in chemotherapy-sensitive group was higher than that in non-control group and drug resistant group(p< 0.05).(4)Retrospective analysis of 100 cases of serous ovarian cancer,and the lymphocyte typing of peripheral blood in patients with tumor control and drug resistance was compared with that in sensitive group.the expression of cCD19+ cells in the sensitive group was higher(p < 0.05).Comparison of lymphocyte typing in peripheral blood of patients with tumor residual ? 1cm and tumor residual < 1cm after operation,the expression level of CD3+?CD3+CD8+ cells was higher in the group of tumor residual < 1cm(p< 0.05).However,there was no significant difference between p53 positive expression group and negative expression group?G1-G2 group and G3-G4 group in different grade of tumor?III group and III-IV group in different stages of tumor.CONCLUSION : The pathological characteristics of serous ovarian cancer with p53 imprinting positive and p53 imprinted negative is different to some extent and there is a certain correlation between p53 imprinting and malignancy of patients with serous ovarian cancer;B lymphocyte(cCD19+)? Ratio of helper lymphocytes to suppressive lymphocyte(Th/Ts)? Total Tlymphocyte(CD3+)? suppressive lymphocyte(CD3+CD8+)in peripheral blood lymphocyte subtype cells may be used for evaluating the condition of serous ovarian cancer tumor patients.